Background Screening for antinuclear antibodies (ANA) by indirect immunofluorescence (IIF) on HEp-2 cells is helpful for the diagnosis and classification of ANA-associated rheumatic diseases, including systemic lupus erythematosus, Sjögren syndrome, mixed connective tissue disease, systemic sclerosis, and inflammatory myopathies. The dense fine speckled (DFS) pattern is a special HEp-2 IIF pattern (produced by anti-DFS70 antibodies) because it is not associated with a specific medical condition and therefore can obfuscate interpretation. Content In this paper, detection methods for and clinical associations of anti-DFS70 antibodies are reviewed. Summary The target antigen of the antibodies that cause the DFS pattern is a 70 kDa protein (DFS70). Commercial methods that detect antibodies to full-length or truncated DFS70 are available for use in clinical laboratories (ELISA, chemiluminescence, dot/line blot). Anti-DFS70 can be found in (apparently) healthy individuals (with a higher frequency in young individuals and in females), in several (inflammatory) conditions and in malignancy. There is no clinical association that is well-established. Special attention (and critical reflection) is given to the observation that monospecific anti-DFS70 (i.e., in the absence of antibodies that are linked to ANA-associated rheumatic diseases) is rarely found in ANA-associated rheumatic diseases.

中文翻译：

---

DFS70 自身抗体：抗核抗体测试的临床实用性


背景 通过 HEp-2 细胞间接免疫荧光 (IIF) 筛选抗核抗体 (ANA) 有助于 ANA 相关风湿性疾病的诊断和分类，包括系统性红斑狼疮、干燥综合征、混合结缔组织病、系统性硬化症和炎症性肌病。密集细斑点 (DFS) 图案是一种特殊的 HEp-2 IIF 图案（由抗 DFS70 抗体产生），因为它与特定的医疗状况无关，因此可能会混淆解释。内容本文对抗DFS70抗体的检测方法及其临床关联进行综述。摘要 导致 DFS 模式的抗体的靶抗原是 70 kDa 蛋白质 (DFS70)。检测全长或截短 DFS70 抗体的商业方法可用于临床实验室（ELISA、化学发光、点/线印迹）。抗 DFS70 存在于（明显）健康个体（在年轻人和女性中出现频率较高）、多种（炎症）病症和恶性肿瘤中。没有明确的临床关联。特别关注（和批判性反思）的是，在 ANA 相关风湿病中很少发现单特异性抗 DFS70（即，在不存在与 ANA 相关风湿病相关的抗体的情况下）的观察结果。