and are widely used models in plant biology research. However, genomic studies of these species have lagged. Here we report the chromosome-level reference genome assemblies for and with an estimated 99.5% and 99.8% completeness, respectively. Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in . Comparative analyses revealed evidence for the parental origins and chromosome structural changes, leading to hybrid genome formation of each species. Interestingly, the antiviral silencing genes , , , , and were lost from one or both subgenomes in , while both homeologs were kept in . Furthermore, the genome encodes fewer immune receptors and signaling components than that of . These findings uncover possible reasons underlying the hypersusceptible nature of . We developed the user-friendly Nicomics () web server to facilitate better use of genomic resources as well as gene structure and expression analyses.

中文翻译：

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高质量组装和注释的烟草和本塞姆氏烟草基因组揭示了染色体进化和防御武器库的变化

是植物生物学研究中广泛使用的模型。然而，这些物种的基因组研究已经滞后。在这里，我们报告了染色体水平参考基因组组装，估计完整性分别为 99.5% 和 99.8%。开发了灵敏的转录起始和终止位点测序方法并用于准确的基因注释。比较分析揭示了亲本起源和染色体结构变化的证据，导致每个物种杂交基因组的形成。有趣的是，抗病毒沉默基因 、 、 、 和 从 中的一个或两个亚基因组中丢失，而 中的两个同源基因均保留。此外，该基因组编码的免疫受体和信号成分比 .这些发现揭示了高度敏感本质背后的可能原因。我们开发了用户友好的 Nicomics () 网络服务器，以促进更好地利用基因组资源以及基因结构和表达分析。