Changes in ambient temperature profoundly affect plant growth and performance. Therefore, the molecular basis of plant acclimation to temperature fluctuation is of great interest. In this study, we discovered that GLYCINE-RICH RNA-BINDING PROTEIN 7 (GRP7) contributes to cold and heat tolerance in . We found that exposure to a warm temperature rapidly induces GRP7 condensates , which can be reversed by transfer to a lower temperature. Cell biology and biochemical assays revealed that GRP7 undergoes liquid–liquid phase separation (LLPS) and LLPS of GRP7 in the cytoplasm contributes to the formation of stress granules that recruit RNA, along with the translation machinery component eukaryotic initiation factor 4E1 (eIF4E1) and the mRNA chaperones COLD SHOCK PROTEIN 1 (CSP1) and CSP3, to inhibit translation. Moreover, natural variations in affecting the residue phosphorylated by the receptor kinase FERONIA alter its capacity to undergo LLPS and correlate with the adaptation of some Arabidopsis accessions to a wider temperature range. Taken together, our findings illustrate the role of translational control mediated by GRP7 LLPS to confer plants with temperature resilience.

中文翻译：

---

FERONIA 介导的磷酸化促进 GRP7 的相分离抑制 mRNA 翻译以调节植物温度恢复能力

环境温度的变化深刻影响植物的生长和性能。因此，植物适应温度波动的分子基础引起了人们的极大兴趣。在这项研究中，我们发现富含甘氨酸的 RNA 结合蛋白 7 (GRP7) 有助于提高 的冷热耐受性。我们发现，暴露在温暖的温度下会迅速产生 GRP7 冷凝物，这可以通过转移到较低的温度来逆转。细胞生物学和生化检测表明，GRP7 经历液-液相分离 (LLPS)，细胞质中 GRP7 的 LLPS 有助于形成招募 RNA 的应激颗粒，以及翻译机制成分真核起始因子 4E1 (eIF4E1) 和mRNA 伴侣冷休克蛋白 1 (CSP1) 和 CSP3，抑制翻译。此外，影响受体激酶 FERONIA 磷酸化残基的自然变化改变了其经历 LLPS 的能力，并与一些拟南芥种质对更宽温度范围的适应相关。总而言之，我们的研究结果说明了 GRP7 LLPS 介导的翻译控制在赋予植物温度适应能力方面的作用。