AimTo evaluate the protein profiles in gingival crevicular fluid (GCF) in relation to clinical outcomes after periodontal surgery and examine if any selected proteins affect the mRNA expression of pro‐inflammatory cytokines in human gingival fibroblasts.Materials and MethodsThis exploratory study included 21 consecutive patients with periodontitis. GCF was collected, and the protein pattern (n = 92) and clinical parameters were evaluated prior to surgery and 3, 6 and 12 months after surgery. Fibroblastic gene expression was analysed by real‐time quantitative polymerase chain reaction.ResultsSurgical treatment reduced periodontal pocket depth (PPD) and changed the GCF protein pattern. Twelve months after surgery, 17% of the pockets showed an increase in PPD. Levels of a number of proteins in the GCF decreased after surgical treatment but increased with early signs of tissue destruction, with LIGHT being one of the proteins that showed the strongest association. Furthermore, LIGHT up‐regulated the mRNA expression of pro‐inflammatory cytokines interleukin (IL)‐6, IL‐8 and MMP9 in human gingival fibroblasts.ConclusionsLIGHT can potentially detect subjects at high risk of periodontitis recurrence after surgical treatment. Moreover, LIGHT induces the expression of inflammatory cytokines and tissue‐degrading enzymes in gingival fibroblasts.

中文翻译：

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LIGHT蛋白：一种新型龈沟液生物标志物，与牙周手术后探诊深度增加相关

目的 评估龈沟液 (GCF) 中的蛋白质谱与牙周手术后临床结果的关系，并检查是否有任何选定的蛋白质影响人牙龈成纤维细胞中促炎细胞因子的 mRNA 表达。 材料和方法这项探索性研究包括 21 名连续患有牙周病的患者。牙周炎。收集GCF，并分析蛋白质模式（n= 92）并在手术前以及手术后 3、6 和 12 个月评估临床参数。通过实时定量聚合酶链反应分析成纤维细胞基因表达。结果手术治疗减少了牙周袋深度（PPD）并改变了 GCF 蛋白模式。手术后 12 个月，17% 的口袋出现 PPD 增加。GCF 中的许多蛋白质水平在手术治疗后下降，但随着组织破坏的早期迹象而增加，其中 LIGHT 是表现出最强关联的蛋白质之一。此外，LIGHT 上调人牙龈成纤维细胞中促炎细胞因子白细胞介素 (IL)-6、IL-8 和 MMP9 的 mRNA 表达。结论 LIGHT 可以潜在地检测手术治疗后牙周炎复发高风险的受试者。此外，光还会诱导牙龈成纤维细胞表达炎症细胞因子和组织降解酶。