SummaryRNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi has a high degree of species‐specificity without impacts on non‐target organisms. However, as plants are inevitably under threat by two or more insect pests in nature, the species‐specific mode of RNAi‐based technology restricts its wide application for pest control. In this study, we artificially designed an intermediate dsRNA (iACT) targeting two β‐Actin (ACT) genes of sap‐sucking pests Bemisia tabaci and Myzus persicae by mutual correction of their mismatches. When expressing hairpin iACT (hpiACT) from tobacco nuclear genome, transgenic plants are well protected from both B. tabaci and M. persicae, either individually or simultaneously, as evidenced by reduced fecundity and suppressed ACT gene expression, whereas expression of hpRNA targeting BtACT or MpACT in transgenic tobacco plants could only confer specific resistance to either B. tabaci or M. persicae, respectively. In sum, our data provide a novel proof‐of‐concept that two different insect species could be simultaneously controlled by artificial synthesis of dsRNA with sequence optimization, which expands the range of transgenic RNAi methods for crop protection.

中文翻译：

---

通过在植物中表达错配校正的双链RNA来控制两种害虫

摘要RNA 干扰 (RNAi) 已成为一种通过沉默目标昆虫的必需基因来控制害虫的有效技术。由于其核苷酸序列引导的工作机制，RNAi具有高度的物种特异性，且不会影响非靶标生物。然而，由于植物在自然界中不可避免地受到两种或两种以上害虫的威胁，基于RNAi的技术的物种特异性模式限制了其在害虫防治中的广泛应用。在本研究中，我们人为设计了一个中间dsRNA（行动计划) 瞄准两个β-肌动蛋白（行为) 吸汁害虫的基因烟粉虱和桃蚜通过相互纠正它们的不匹配。表达发夹时行动计划（生命值行动计划）从烟草核基因组来看，转基因植物受到了很好的保护烟粉虱和桃蚜，单独或同时，如繁殖力降低和抑制所证明行为基因表达，而hpRNA靶向表达BTACT或者MpACT在转基因烟草植物中只能赋予对其中任何一种的特异性抗性烟粉虱或者桃蚜， 分别。总之，我们的数据提供了一种新的概念验证，即可以通过人工合成 dsRNA 并进行序列优化来同时控制两种不同的昆虫物种，从而扩大了作物保护转基因 RNAi 方法的范围。