Plants have an astonishing ability to regenerate new organs after wounding. Here, we report that the wound-inducible transcription factor ENHANCER OF SHOOT REGENERATION1 (ESR1) has a dual mode of action in activating ANTHRANILATE SYNTHASE ALPHA SUBUNIT1 (ASA1) expression to ensure auxin-dependent de novo root organogenesis locally at wound sites of Arabidopsis (Arabidopsis thaliana) leaf explants. In the first mode, ESR1 interacts with HISTONE DEACETYLASE6 (HDA6), and the ESR1–HDA6 complex directly binds to the JASMONATE-ZIM DOMAIN5 (JAZ5) locus, inhibiting JAZ5 expression through histone H3 deacetylation. As JAZ5 interferes with the action of ETHYLENE RESPONSE FACTOR109 (ERF109), the transcriptional repression of JAZ5 at the wound site allows ERF109 to activate ASA1 expression. In the second mode, the ESR1 transcriptional activator directly binds to the ASA1 promoter to enhance its expression. Overall, our findings indicate that the dual biochemical function of ESR1, which specifically occurs near wound sites of leaf explants, maximizes local auxin biosynthesis and de novo root organogenesis in Arabidopsis.

中文翻译：

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芽再生增强剂1促进拟南芥叶外植体受伤后根的器官发生

植物在受伤后具有惊人的再生新器官的能力。在此，我们报道伤口诱导转录因子 ENHANCER OF SHOOT REGENERATION1 (ESR1) 在激活邻氨基苯甲酸盐合成酶 ALPHA 亚单位 (ASA1) 表达方面具有双重作用模式，以确保拟南芥伤口部位局部生长素依赖性从头器官发生。拟南芥）叶外植体。在第一种模式中，ESR1 与组蛋白去乙酰化酶 6 (HDA6) 相互作用，ESR1-HDA6 复合物直接与 JASMONATE-ZIM DOMAIN5 (JAZ5) 位点结合，通过组蛋白 H3 去乙酰化抑制 JAZ5 表达。由于 JAZ5 干扰乙烯反应因子 109 (ERF109) 的作用，伤口部位 JAZ5 的转录抑制使 ERF109 激活 ASA1 表达。在第二种模式中，ESR1转录激活因子直接结合ASA1启动子以增强其表达。总的来说，我们的研究结果表明，ESR1 的双重生化功能（特别发生在叶子外植体的伤口部位附近）最大化了拟南芥中的局部生长素生物合成和从头根器官发生。