Self-assembled monolayers (SAMs) represent highly ordered molecular materials with versatile biochemical features and multidisciplinary applications. Research on SAMs has made much progress since the early begginings of Au substrates and alkanethiols, and numerous examples of peptide-displaying SAMs can be found in the literature. Peptides, presenting increasing structural complexity, stimuli-responsiveness, and biological relevance, represent versatile functional components in SAMs-based platforms. This review examines the major findings and progress made on the use of peptide building blocks displayed as part of SAMs with specific functions, such as selective cell adhesion, migration and differentiation, biomolecular binding, advanced biosensing, molecular electronics, antimicrobial, osteointegrative and antifouling surfaces, among others. Peptide selection and design, functionalisation strategies, as well as structural and functional characteristics from selected examples are discussed. Additionally, advanced fabrication methods for dynamic peptide spatiotemporal presentation are presented, as well as a number of characterisation techniques. All together, these features and approaches enable the preparation and use of increasingly complex peptide-based SAMs to mimic and study biological processes, and provide convergent platforms for high throughput screening discovery and validation of promising therapeutics and technologies.

中文翻译：

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基于肽的自组装单层 (SAM)：肽对 SAM 有何作用，反之亦然

自组装单层 (SAM) 代表高度有序的分子材料，具有多种生化特征和多学科应用。自从金底物和链烷硫醇的早期开始以来，SAM 的研究已经取得了很大进展，并且在文献中可以找到大量肽展示 SAM 的例子。肽呈现出不断增加的结构复杂性、刺激响应性和生物相关性，代表了基于 SAM 的平台中的多功能功能组件。本综述探讨了使用肽构建块的主要发现和进展，这些肽构建块显示为具有特定功能的 SAM 的一部分，例如选择性细胞粘附、迁移和分化、生物分子结合、先进生物传感、分子电子学、抗菌、骨整合和防污表面等。讨论了肽的选择和设计、功能化策略以及所选示例的结构和功能特征。此外，还介绍了动态肽时空呈现的先进制造方法以及许多表征技术。总之，这些特征和方法使得能够制备和使用日益复杂的基于肽的 SAM 来模拟和研究生物过程，并为高通量筛选发现和验证有前途的疗法和技术提供聚合平台。