AimTo evaluate the effect of subgingival delivery of progranulin (PGRN)/gelatin methacryloyl (GelMA) complex as an adjunct to scaling and root planing (SRP) on an experimental periodontitis dog model with Class II furcation involvement (FI).Materials and MethodsA Class II FI model was established, and the defects were divided into four treatment groups: (a) no treatment (control); (b) SRP; (c) SRP + GelMA; (d) SRP + PGRN/GelMA. Eight weeks after treatment, periodontal parameters were recorded, gingival crevicular fluid and gingival tissue were collected for ELISA and RT‐qPCR, respectively, and mandibular tissue blocks were collected for micro computed tomography (micro‐CT) scanning and hematoxylin and eosin (H&E) staining.ResultsThe SRP + PGRN/GelMA group showed significant improvement in all periodontal parameters compared with those in the other groups. The expression of markers related to M1 macrophage and Th17 cell significantly decreased, and the expression of markers related to M2 macrophage and Treg cell significantly increased in the SRP + PGRN/GelMA group compared with those in the other groups. The volume, quality and area of new bone and the length of new cementum in the root furcation defects of the PGRN/GelMA group were significantly increased compared to those in the other groups.ConclusionsSubgingival delivery of the PGRN/GelMA complex could be a promising non‐surgical adjunctive therapy for anti‐inflammation, immunomodulation and periodontal regeneration.

中文翻译：

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龈下输送的颗粒体蛋白前体水凝胶作为狗 II 类分叉受累非手术治疗的辅助手段增强了免疫调节和牙周再生功效

目的 评估龈下递送颗粒体蛋白前体 (PGRN)/明胶甲基丙烯酰 (GelMA) 复合物作为洁治和根面平整 (SRP) 辅助手段对 II 类分叉受累 (FI) 的实验性牙周炎犬模型的效果。 材料和方法A II 类建立FI模型，将缺陷分为四个治疗组：（a）不治疗（对照）；(b) 建议调整计划；(c) SRP + GelMA；(d) SRP + PGRN/GelMA。治疗后八周，记录牙周参数，收集龈沟液和牙龈组织分别进行ELISA和RT-qPCR，收集下颌组织块进行显微计算机断层扫描（micro-CT）和苏木精和伊红（H&E）结果SRP+PGRN/GelMA组各项牙周参数较其他组均有显着改善。与其他组相比，SRP+PGRN/GelMA组M1巨噬细胞和Th17细胞相关标志物表达显着降低，M2巨噬细胞和Treg细胞相关标志物表达显着升高。与其他组相比，PGRN/GelMA 组牙根分叉缺损处新骨的体积、质量和面积以及新牙骨质的长度均显着增加。结论 PGRN/GelMA 复合体的龈下递送可能是一种有前途的非修复方法。 ‐抗炎、免疫调节和牙周再生的手术辅助治疗。