AimTo evaluate the effect of non‐surgical periodontal therapy (NSPT) on salivary histone deacetylases (HDACs) gene expression in patients with Stage III–IV periodontitis at baseline and at 3 and 6 months post NSPT treatment.Materials and MethodsTwenty patients completed the study. Periodontitis (as well as the corresponding staging and grading) was diagnosed according to the 2017 World Workshop Classification. Clinical measures were recorded and whole unstimulated saliva was collected at baseline and at 3 and 6 months after NSPT. The expression of 11 HDACs was determined using reverse‐transcription PCR, and the respective changes over time were evaluated.ResultsSix months after NSPT, significant improvements in all clinical periodontal parameters were observed, concomitant with significant up‐regulation of HDAC2, 4, 6, 8, 9 and 11 expressions. Subgroup analyses of non‐responders and responders revealed no significant differences in HDACs mRNA expression between groups at any time point.ConclusionsThis prospective clinical study identified longitudinal changes in salivary HDACs expression in response to NSPT, which provides new insights into the epigenetic mechanisms underlying the pathobiology of periodontitis and creates avenues for the discovery of novel biomarkers.

中文翻译：

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非手术牙周治疗对唾液腺组蛋白脱乙酰酶表达的影响：一项前瞻性临床研究

目的评价非手术牙周治疗（NSPT）对唾液腺的影响组蛋白脱乙酰酶 (HDAC)III-IV 期牙周炎患者在基线以及 NSPT 治疗后 3 个月和 6 个月时的基因表达。材料和方法 20 名患者完成了这项研究。牙周炎（以及相应的分期和分级）根据2017年世界研讨会分类进行诊断。记录临床测量结果，并在基线以及 NSPT 后 3 个月和 6 个月收集完整的未刺激唾液。11的表达式组蛋白去乙酰化酶使用逆转录 PCR 测定，并评估随时间的各自变化。 结果 NSPT 六个月后，观察到所有临床牙周参数显着改善，同时HDAC2, 4, 6, 8, 9和11表达式。对无反应者和反应者的亚组分析显示，在组蛋白去乙酰化酶任何时间点各组之间的 mRNA 表达。结论这项前瞻性临床研究确定了唾液的纵向变化组蛋白去乙酰化酶NSPT 的表达，这为牙周炎病理学背后的表观遗传机制提供了新的见解，并为发现新的生物标志物创造了途径。