BackgroundThe aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar‐incisor pattern periodontitis (C‐MIP) affecting the primary or permanent dentitions.MethodsDNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C‐MIP patients and subjected to phylogenetic microarray analysis. C‐MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within‐subject differences between C‐MIP‐affected sites and dentition‐matched healthy sites were also evaluated.ResultsC‐MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C‐MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C‐MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C‐MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C‐MIP sites. Moreover, both permanent and primary C‐MIP sites showed distinct microbial communities when compared to dentition‐matched healthy sites in the same subject (p < 0.01).ConclusionsPrimary and permanent teeth with C‐MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C‐MIP in both dentitions.

中文翻译：

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青少年 C 级牙周炎乳牙和恒牙列微生物群的评估

背景本研究的目的是评估影响乳牙或恒牙列的 C 级磨牙切牙型牙周炎 (C-MIP) 患者的龈下微生物组。方法从 45 C- 患病部位和健康部位的龈下生物膜样本中分离 DNA。 MIP 患者并进行系统发育微阵列分析。对初生牙列受影响的儿童和恒牙列受影响的儿童之间的 C-MIP 位置进行了比较。还评估了 C-MIP 受影响部位和牙列匹配健康部位之间的受试者内差异。结果，乳牙列受影响受试者的 C-MIP 部位显示出与 C-MIP 恒牙列部位部分重叠但不同的微生物群落（p< 0.05）。差异是由于该属某些物种的初级 C-MIP 位点水平增加所致噬二氧化碳菌门和细毛菌门，而 C-MIP 恒牙列位点显示出更高的患病率毛丝因子。放线菌伴生聚集菌（氨基酸）是在主要和永久 C-MIP 地点中流行率和水平都很高的物种之一。此外，与同一受试者中牙列匹配的健康部位相比，永久和主要 C-MIP 部位都显示出不同的微生物群落（p< 0.01)。结论 C-MIP 的乳牙和恒牙显示出微生物群失调，乳牙列受影响的儿童与恒牙列受影响的儿童表现出不同的轮廓。然而，氨基酸在原发性和永久性病变部位均富集，证实该微生物与两个牙列的 C-MIP 有关。