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Effects of high-pressure homogenization and ultrasound on the composition, structure, and physicochemical properties of proteins extracted from Nannochloropsis Oceania
Ultrasonics Sonochemistry ( IF 8.4 ) Pub Date : 2024-03-20 , DOI: 10.1016/j.ultsonch.2024.106851
Wee Jian Cedric Sow , Juan Du

This study examined the effects of high-pressure homogenization (HPH) and ultrasonication pre-treatment on the structural and physicochemical properties of proteins extracted from defatted biomass (DNOB). HPH treatment was found to enhance the solubility of protein extracted from DNOB compared to ultrasound, where samples pretreated with three passes (3P) of HPH exhibited lower solubility than two passes (2P). The morphology of extracted samples was visualized by scanning electron microscopy, which HPH pre-treatment, especially with more passes, were able to breakdown DNOB into fragments. Alternatively, more holes were displayed on the surface of the extracts pretreated with ultrasound especially when higher amplitude applied. The particle size of extracts from HPH (129.5 µm) significant dropped from HPH (314.25 µm), where samples pretreated with ultrasound at 20 % amplitude (US) also decreased in particle size compared to 40 % amplitude (US), from 115.25 µm to 78.22 µm. Protein flexibility of DNOB extracts were enhanced by both HPH and HPH but decreased for ultrasound samples. β-sheets were found to be the most abundant protein secondary structure for all samples, where samples treated with HPH contained the highest percentage of β-sheets (72 %) than control, HPH, ultrasonication at 20 and 40 % amplitude (52–62 %). The high percentage of β-sheets found in HPH sample also contributed to its outstanding emulsifying properties which stood out among all, especially at concentrations over 1 mg/ml. Results obtained from this study helped to direct the application of DNOB extracts as functional food ingredient for future food innovation.

中文翻译:


高压均质和超声对大洋洲微拟球藻提取蛋白质组成、结构和理化性质的影响



本研究探讨了高压均质化 (HPH) 和超声波预处理对从脱脂生物质 (DNOB) 中提取的蛋白质的结构和理化性质的影响。研究发现,与超声波相比,HPH 处理可提高从 DNOB 中提取的蛋白质的溶解度,其中经过三遍 (3P) HPH 预处理的样品表现出比两遍 (2P) 更低的溶解度。提取样品的形态通过扫描电子显微镜观察,HPH 预处理,尤其是多次处理,能够将 DNOB 分解成碎片。或者,用超声波预处理的提取物表面上显示出更多的孔,特别是当施加更高的振幅时。 HPH 提取物的粒径 (129.5 µm) 较 HPH (314.25 µm) 显着下降,与 40% 振幅 (US) 相比,用 20% 振幅 (US) 超声波预处理的样品粒径也从 115.25 µm 下降到78.22 微米。 HPH 和 HPH 均增强了 DNOB 提取物的蛋白质灵活性,但超声样品的蛋白质灵活性有所降低。 β-折叠被发现是所有样品中最丰富的蛋白质二级结构,其中用 HPH 处理的样品比对照、HPH、20 和 40% 振幅超声处理 (52-62 %)。 HPH 样品中发现的高比例 β-折叠也使其具有突出的乳化性能,尤其是在浓度超过 1 mg/ml 时。这项研究获得的结果有助于指导 DNOB 提取物作为功能性食品成分的应用,以促进未来的食品创新。
更新日期:2024-03-20
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