Summary: The transition from 2D to 3D spatial profiling marks a revolutionary era in cancer research, offering unprecedented potential to enhance cancer diagnosis and treatment. This commentary outlines the experimental and computational advancements and challenges in 3D spatial molecular profiling, underscoring the innovation needed in imaging tools, software, artificial intelligence, and machine learning to overcome implementation hurdles and harness the full potential of 3D analysis in the field.

中文翻译：

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癌症发现的 3D 革命

摘要：从 2D 空间分析到 3D 空间分析的转变标志着癌症研究的革命性时代，为增强癌症诊断和治疗提供了前所未有的潜力。本评论概述了 3D 空间分子分析中的实验和计算进展以及挑战，强调了成像工具、软件、人工智能和机器学习所需的创新，以克服实施障碍并充分利用该领域 3D 分析的潜力。