Advances in surface-enhanced Raman scattering (SERS) detection have helped to overcome the limitations of traditional in vitro diagnostic methods, such as fluorescence and chemiluminescence, owing to its high sensitivity and multiplex detection capability. However, for the implementation of SERS detection technology in disease diagnosis, a SERS-based assay platform capable of analyzing clinical samples is essential. Moreover, infectious diseases like COVID-19 require the development of point-of-care (POC) diagnostic technologies that can rapidly and accurately determine infection status. As an effective assay platform, SERS-based bioassays utilize SERS nanotags labeled with protein or DNA receptors on Au or Ag nanoparticles, serving as highly sensitive optical probes. Additionally, a microdevice is necessary as an interface between the target biomolecules and SERS nanotags. This review aims to introduce various microdevices developed for SERS detection, available for POC diagnostics, including LFA strips, microfluidic chips, and microarray chips. Furthermore, the article presents research findings reported in the last 20 years for the SERS-based bioassay of various diseases, such as cancer, cardiovascular diseases, and infectious diseases. Finally, the prospects of SERS bioassays are discussed concerning the integration of SERS-based microdevices and portable Raman readers into POC systems, along with the utilization of artificial intelligence technology.

中文翻译：

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基于 SERS 的微器件用作体外诊断生物传感器

表面增强拉曼散射（SERS）检测的进步凭借其高灵敏度和多重检测能力，有助于克服荧光和化学发光等传统体外诊断方法的局限性。然而，为了将SERS检测技术应用于疾病诊断，一个能够分析临床样本的基于SERS的检测平台是必不可少的。此外，像 COVID-19 这样的传染病需要开发能够快速准确地确定感染状态的护理点 (POC) 诊断技术。作为一种有效的检测平台，基于 SERS 的生物检测利用 Au 或 Ag 纳米颗粒上标记有蛋白质或 DNA 受体的 SERS 纳米标签，作为高灵敏度的光学探针。此外，还需要微型设备作为目标生物分子和 SERS 纳米标签之间的接口。本综述旨在介绍为SERS检测开发的各种可用于POC诊断的微器件，包括LFA条、微流控芯片和微阵列芯片。此外，本文还介绍了过去 20 年基于 SERS 的多种疾病生物测定的研究成果，如癌症、心血管疾病和传染病。最后，讨论了 SERS 生物测定的前景，涉及将基于 SERS 的微型设备和便携式拉曼读数器集成到 POC 系统中，以及人工智能技术的利用。