当前位置: X-MOL 学术ISME J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Anaerobic oxidation of ammonium and short-chain gaseous alkanes coupled to nitrate reduction by a bacterial consortium
The ISME Journal ( IF 11.0 ) Pub Date : 2024-04-16 , DOI: 10.1093/ismejo/wrae063
Mengxiong Wu 1 , Xiawei Liu 1 , J Pamela Engelberts 2, 3 , Gene W Tyson 2, 3 , Simon J McIlroy 2, 3 , Jianhua Guo 1
Affiliation  

The bacterial species ‘Candidatus Alkanivorans nitratireducens’ was recently demonstrated to mediate nitrate-dependent anaerobic oxidation of short-chain gaseous alkanes (SCGAs). In previous bioreactor enrichment studies1,2, the species appeared to reduce nitrate in two phases, switching from denitrification to dissimilatory nitrate reduction to ammonium (DNRA) in response to nitrite accumulation. The regulation of this switch or the nature of potential syntrophic partnerships with other microorganisms remains unclear. Here, we describe anaerobic multispecies cultures of bacteria which couple the oxidation of propane and butane to nitrate reduction and the oxidation of ammonium (anammox). Batch tests with 15N-isotope labelling and multi-omic analyses collectively supported a syntrophic partnership between ‘Ca. A. nitratireducens’ and anammox bacteria, with the former species mediating nitrate-driven oxidation of SCGAs, supplying the latter with nitrite for the oxidation of ammonium. The elimination of nitrite accumulation by the anammox substantially increased SCGA and nitrate consumption rates, whereas suppressing DNRA. Removing ammonium supply led to its eventual production, the accumulation of nitrite, and the upregulation of DNRA gene expression for the abundant ‘Ca. A. nitratireducens’. Increasing the supply of SCGA had a similar effect in promoting DNRA. Our results suggest that ‘Ca. A. nitratireducens’ switches to DNRA to alleviate oxidative stress caused by nitrite accumulation, giving further insight into adaptability and ecology of this microorganism. Our findings also have important implications for the understanding of the fate of nitrogen and SCGAs in anaerobic environments.

中文翻译:

铵和短链气态烷烃的厌氧氧化与细菌群落的硝酸盐还原相结合

最近证明细菌物种“Candidatus Alkanivorans nittireducens”可以介导短链气态烷烃(SCGA)的硝酸盐依赖性厌氧氧化。在之前的生物反应器富集研究1,2中,该物种似乎分两个阶段还原硝酸盐,响应亚硝酸盐积累,从反硝化转变为异化硝酸盐还原为铵(DNRA)。这种开关的调节或与其他微生物潜在的互养伙伴关系的性质仍不清楚。在这里,我们描述了厌氧多物种细菌培养物,其将丙烷和丁烷的氧化与硝酸盐还原和铵(厌氧氨氧化)的氧化结合起来。 15N 同位素标记和多组学分析的批量测试共同支持了 'Ca.硝基还原菌和厌氧氨氧化菌,前一种细菌介导硝酸盐驱动的 SCGA 氧化,为后者提供亚硝酸盐以氧化铵。厌氧氨氧化消除亚硝酸盐积累显着增加了 SCGA 和硝酸盐的消耗率,同时抑制了 DNRA。去除铵供应导致其最终产生、亚硝酸盐积累以及丰富的 'Ca 的 DNRA 基因表达上调。 A. 硝基还原菌'。增加 SCGA 的供应对于促进 DNRA 具有类似的效果。我们的结果表明'Ca。 A. nittireducens 转而使用 DNRA 来减轻亚硝酸盐积累引起的氧化应激,从而进一步了解该微生物的适应性和生态学。我们的研究结果对于理解厌氧环境中氮和 SCGA 的命运也具有重要意义。
更新日期:2024-04-16
down
wechat
bug