Decellularized animal tissue may serve as scaffolds in tissue engineering. Despite the well-disseminated use of sodium dodecyl sulfate (SDS) for decellularization, SDS-based methods are time-consuming and may cause extracellular matrix (ECM) denaturation. This study explores supercritical CO (scCO) extraction as an alternative to decellularize chicken sternum cartilage. Various scCO decellularization methods were tested, including pure scCO, scCO with water, ethanol, or their mixture. SDS outperformed in decellularization but caused the highest extracellular matrix damage. Pure scCO treatment for 2 h reduced mechanical resistance due to dehydration. scCO with 99 % ethanol led to extensive tissue dehydration, while the addition of 70 % ethanol increased cell removal without significant tissue damage. Further research is recommended to optimize scCO decellularization of this tissue with other cosolvents while maintaining adequate hydration.

中文翻译：

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超临界软骨脱细胞：共溶剂对机械性能的影响

脱细胞动物组织可作为组织工程中的支架。尽管使用十二烷基硫酸钠 (SDS) 进行脱细胞化已得到广泛应用，但基于 SDS 的方法非常耗时，并且可能导致细胞外基质 (ECM) 变性。本研究探索超临界 CO (scCO) 萃取作为鸡胸骨软骨脱细胞的替代方法。测试了各种 scCO 脱细胞方法，包括纯 scCO、scCO 与水、乙醇或其混合物。 SDS 在脱细胞化方面表现出色，但造成的细胞外基质损伤最高。纯 scCO 处理 2 小时，由于脱水而降低了机械阻力。含有 99% 乙醇的 scCO 会导致广泛的组织脱水，而添加 70% 乙醇可增加细胞去除率，而不会造成明显的组织损伤。建议进一步研究以优化该组织与其他共溶剂的 scCO 脱细胞作用，同时保持足够的水合作用。