The recent COVID-19 pandemic has underscored the need for innovative decontamination techniques capable of treating sensitive materials potentially contaminated. Combining supercritical carbon dioxide (scCO) with sterilant agents has shown promise in this regard. This study aimed at testing scCO as a virus inactivation method for biomedical materials contaminated with the Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). The virus was inoculated on a stainless-steel carrier and treated at 45 °C and 8 MPa. No inactivation was detected when only scCO was used, even after long treatment times (60 min). The addition of 50 ppm of HO to the process allowed the inactivation of more than 5 Log PFU (Plaque Forming Unit) of the virus by only pressurising and depressurising the vessel, while a 20-min process is needed by only using HO. Overall, the study demonstrates a synergistic effect when HO is added to the scCO process for the inactivation of SARS-CoV-2.

中文翻译：

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超临界二氧化碳与过氧化氢结合灭活 SARS-CoV-2

最近的 COVID-19 大流行凸显了对能够处理可能受到污染的敏感材料的创新净化技术的需求。将超临界二氧化碳 (scCO) 与灭菌剂相结合在这方面已显示出前景。本研究旨在测试 scCO 作为一种病毒灭活方法，用于治疗受严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 污染的生物医学材料。将病毒接种在不锈钢载体上，并在45℃和8MPa下处理。当仅使用 scCO 时，即使经过较长的处理时间（60 分钟），也未检测到失活。在该过程中添加 50 ppm H2O，仅通过对容器加压和减压即可灭活超过 5 Log PFU（空斑形成单位）的病毒，而仅使用 H2O 则需要 20 分钟的过程。总体而言，该研究表明，当将 H2O2 添加到 scCO 过程中时，可产生协同效应以灭活 SARS-CoV-2。