The rhizosphere is one of the key determinants of plant health and productivity. Mixtures of pesticides are commonly used in intensified agriculture. However, the combined mechanisms underlying their impacts on soil microbiota remain unknown. The present study revealed that the rhizosphere microbiota was more sensitive to azoxystrobin and oxytetracycline, two commonly used pesticides, than was the microbiota present in bulk soil. Moreover, the rhizosphere microbiota enhanced network complexity and stability and increased carbohydrate metabolism and xenobiotic biodegradation as well as the expression of metabolic genes involved in defence against pesticide stress. Co-exposure to azoxystrobin and oxytetracycline had antagonistic effects on growth and soil microbial variation by recruiting organic-degrading bacteria and regulating ABC transporters to reduce pesticide uptake. Our study explored the composition and function of soil microorganisms through amplicon sequencing and metagenomic approaches, providing comprehensive insights into the synergistic effect of plants and rhizosphere microbiota on pesticides and contributing to our understanding of the ecological risks associated with pesticide use.

中文翻译：

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嘧菌酯和土霉素对拟南芥根际微生物的联合影响


根际是植物健康和生产力的关键决定因素之一。农药混合物常用于集约化农业。然而，它们对土壤微生物群影响的综合机制仍然未知。本研究表明，根际微生物群对嘧菌酯和土霉素这两种常用农药比大块土壤中的微生物群更敏感。此外，根际微生物群增强了网络的复杂性和稳定性，增加了碳水化合物代谢和外源生物降解，以及参与防御农药胁迫的代谢基因的表达。同时接触嘧菌酯和土霉素对生长和土壤微生物变异产生拮抗作用，通过招募有机降解细菌和调节 ABC 转运蛋白来减少农药的吸收。我们的研究通过扩增子测序和宏基因组方法探索了土壤微生物的组成和功能，为植物和根际微生物群对农药的协同效应提供了全面的见解，并有助于我们了解与农药使用相关的生态风险。