The paper demonstrates an adaptation of a 3D printer (Prusa Mini+) with novel modules to develop a droplet generation system that generates combinatorial droplets from a standard 96 well plate. The calibration methodology developed would allow any fused deposition modeling (FDM) printer to generate monodisperse droplets (coefficient of variance (CV%) < 5%) from well plates or vials of any geometry. The system maintains precision across various volumes while maintaining a C.V. range of 0.81% to 3.61%, with an increased precision for larger volumes. The cost of the system developed is 70% less than commercially available droplet generation packages. Successful droplet library storage is accomplished via 3D printed cartridge connectors. The implemented system has been calibrated for Tygon® and PTFE at different velocities and volumetric configurations.

中文翻译：

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自动动态入口微流体系统：3D 打印机适应经济高效、小批量、按需多分析物液滴发生器

该论文展示了 3D 打印机 (Prusa Mini+) 与新颖模块的配合，以开发液滴生成系统，该系统可从标准 96 孔板生成组合液滴。开发的校准方法将允许任何熔融沉积建模 (FDM) 打印机从任何几何形状的孔板或小瓶生成单分散液滴（方差系数 (CV%) < 5%）。该系统可在不同体积下保持精度，同时保持 0.81% 至 3.61% 的 CV 范围，并针对较大体积提高精度。所开发系统的成本比市售液滴生成包低 70%。成功的液滴库存储是通过3D 打印的墨盒连接器完成的。所实施的系统已针对不同速度和体积配置下的 Tygon® 和 PTFE 进行了校准。