Aphids (Hemiptera: Aphididae) are notorious sap-sucking insects that rampantly threaten agricultural production worldwide. Current management against aphids in the field heavily relies on chemical pesticides, which makes economical and eco-friendly methods urgently needed. Spray-induced gene silencing (SIGS) offers a powerful and precise approach to pest management. However, the high costs and instability of double-stranded RNA (dsRNA) regulators applied for downstream RNA interference (RNAi) still limit this strategy. It remains uncertain if RNAi regulators applied in SIGS could extend to small RNA (sRNA), especially miRNA.

中文翻译：

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植物来源的人工 miRNA 通过喷雾诱导基因沉默有效减少蚜虫 (Aphidoidea) 的增殖

蚜虫（半翅目：蚜科）是臭名昭著的吸汁昆虫，严重威胁着全世界的农业生产。目前田间蚜虫防治严重依赖化学农药，迫切需要经济环保的方法。喷雾诱导基因沉默（SIGS）为害虫管理提供了一种强大而精确的方法。然而，用于下游RNA干扰（RNAi）的双链RNA（dsRNA）调节剂的高成本和不稳定性仍然限制了这一策略。目前尚不确定 SIGS 中应用的 RNAi 调节剂是否可以扩展到小 RNA (sRNA)，尤其是 miRNA。