Specific detection of glycoproteins such as transferrin (TRF) related to neurological diseases, hepatoma and other diseases always plays an important role in the field of disease diagnosis. We designed an antibody-free immunoassay sensing method based on molecularly imprinted polymers (MIPs) formed by the polymerization of multiple functional monomers for the sensitive and selective detection of TRF in human serum. In the sandwich surface-enhanced Raman spectroscopy (SERS) sensor, the TRF-oriented magnetic MIP nanoparticles (Fe₃O₄@SiO₂–MIPs) served as capture units to specifically recognize TRF and 4-mercaptophenylboronic acid-functionalized gold nanorods (MPBA–Au NRs) served as SERS probes to label the targets. In order to achieve stronger interaction between the recognition cavities of the prepared MIPs and the different amino acid fragments that make up TRF, Fe₃O₄@SiO₂–MIPs were obtained through polycondensation reactions between more silylating reagents, enhancing the specific recognition of the entire TRF protein and achieving high IF. This sensing method exhibited a good linear response to TRF within the TRF concentration range of 0.01 ng mL⁻¹ to 1 mg mL⁻¹ (R² = 0.9974), and the LOD was 0.00407 ng mL⁻¹ (S/N = 3). The good stability, reproducibility and specificity of the resulting MIP based SERS sensor were demonstrated. The determination of TRF in human serum confirmed the feasibility of the method in practical applications.

中文翻译：

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基于分子印迹聚合物的 SERS 传感人血清中的转铁蛋白

与神经系统疾病、肝癌等疾病相关的转铁蛋白（TRF）等糖蛋白的特异性检测一直在疾病诊断领域发挥着重要作用。我们设计了一种基于由多个功能单体聚合形成的分子印迹聚合物（MIP）的无抗体免疫测定传感方法，用于灵敏、选择性地检测人血清中的TRF。在三明治表面增强拉曼光谱（SERS）传感器中，TRF取向的磁性MIP纳米颗粒（Fe ₃ O ₄ @SiO ₂ –MIPs）作为捕获单元来特异性识别TRF和4-巯基苯硼酸功能化金纳米棒（MPBA） –Au NR）用作 SERS 探针来标记目标。为了使所制备的MIPs的识别腔与构成TRF的不同氨基酸片段之间产生更强的相互作用，通过更多的甲硅烷基化试剂之间的缩聚反应获得了Fe ₃ O ₄ @SiO ₂ –MIPs，增强了对MIPs的特异性识别。完整的 TRF 蛋白并实现高 IF。该传感方法在TRF浓度范围0.01 ng mL ^-1至1 mg mL ^-1 ( R ² = 0.9974)内表现出良好的TRF线性响应，LOD为0.00407 ng mL ^-1 (S/N = 3) 。所得到的基于 MIP 的 SERS 传感器具有良好的稳定性、重现性和特异性。人血清中TRF的测定证实了该方法在实际应用中的可行性。