The sensing of small molecules is important to a variety of fields and particularly to clinical diagnostics. Estriol is an important small chemical molecule, as it is one of three markers measured during mid‐gestation for the Down syndrome. Point‐of‐care testing of estriol will increase maternal compliance for aneuploidy mid‐gestation screening. A bioFET technology is a promising point‐of‐care platform. The meta‐nano‐channel (MNC) bioFET is employed with its tunable channel configuration to optimize the coupling between the electrostatics of the estriol‐receptor interactions and the electrodynamics of the conducting readout current. Also, the MNC bioFET receptor layer is composed of anti‐estriol antibodies to address the high structural similarity of estriol to other estrogens. Despite the small size and the electrical neutrality of estriol, real‐time is demonstrated, specific, and label‐free sensing of estriol in 0.5 µL of 1:100 diluted plasma with excellent linearity, sensitivity, dynamic range extending to ten orders of magnitude in estriol concentration and limit‐of‐detection of 1 fg mL−1. Extensive control and non‐specific measurements are reported including the response to other neutral members of the estrogen family.

中文翻译：

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对微升稀释血浆中电中性小雌三醇分子与其抗体之间的相互作用进行特异性且无标记的生物场效应晶体管 (bioFET) 传感

小分子的传感对于许多领域都很重要，特别是对于临床诊断。雌三醇是一种重要的小化学分子，因为它是在妊娠中期测量的唐氏综合症的三个标志物之一。雌三醇的护理点检测将提高母亲对非整倍体妊娠中期筛查的依从性。 BioFET 技术是一种很有前景的护理点平台。元纳米通道（MNC）bioFET 以其可调谐通道配置来优化雌三醇受体相互作用的静电与传导读出电流的电动力学之间的耦合。此外，MNC bioFET 受体层由抗雌三醇抗体组成，以解决雌三醇与其他雌激素结构高度相似的问题。尽管雌三醇尺寸小且呈电中性，但在 0.5 µL 1:100 稀释血浆中对雌三醇进行了实时、特异且无标记的传感，具有出色的线性度、灵敏度，动态范围可扩展至十个数量级雌三醇浓度和检测限为 1 fg mL−1。据报道，广泛的控制和非特异性测量包括对雌激素家族其他中性成员的反应。