The multicellular trichomes of cucumber ( L.) serve as the primary defense barrier against external factors, whose impact extends beyond plant growth and development to include commercial characteristics of fruits. The aphid ( Glover) is one of prominent pests in cucumber cultivation. However, the relationship between physical properties of trichomes and the aphid resistance at molecular level remains largely unexplored. Here, a spontaneous mutant () was characterized by increased susceptibility towards aphid. Further observations showed the exhibited a higher and narrower trichome base, which was significantly distinguishable from that in wild-type (WT). We conducted map-based cloning and identified the candidate, , encoding a C-lectin receptor-like kinase. The knockout mutant demonstrated the role of in trichome morphogenesis. The presence of SNP does not regulate the relative expression of but diminishes the CsTM abundance of membrane proteins in . Interestingly, CsTM was found to interact with CsTIP1;1, which encodes an aquaporin with extensive reports in plant resistance and growth development. The subsequent aphid resistance experiments revealed that both and regulated the development of trichomes and conferred resistance against aphid by affecting cytoplasmic HO contents. Transcriptome analysis revealed a significant enrichment of genes associated with pathogenesis, calcium binding and cellulose synthase. Overall, our study elucidates an unidentified mechanism that alters multicellular trichome morphology and enhances resistance against aphid, thus providing a wholly new perspective for trichome morphogenesis in cucumber.

中文翻译：

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CsTM 通过与黄瓜中的 CsTIP1;1 相互作用改变多细胞毛状体形态并增强对蚜虫的抵抗力

黄瓜 (L.) 的多细胞毛状体是抵御外部因素的主要防御屏障，其影响不仅限于植物生长和发育，还包括水果的商业特性。蚜虫（Glover）是黄瓜栽培中的主要害虫之一。然而，毛状体的物理特性与分子水平上的蚜虫抗性之间的关系在很大程度上仍未被探索。在此，自发突变体（）的特征是对蚜虫的敏感性增加。进一步观察显示，其毛状体碱基更高、更窄，与野生型（WT）有显着区别。我们进行了基于图谱的克隆并鉴定了编码 C-凝集素受体样激酶的候选物 。敲除突变体证明了在毛状体形态发生中的作用。 SNP 的存在不会调节 的相对表达，但会减少 中膜蛋白的 CsTM 丰度。有趣的是，CsTM被发现与CsTIP1;1相互作用，CsTIP1;1编码水通道蛋白，在植物抗性和生长发育方面有广泛的报道。随后的蚜虫抗性实验表明， 和 都调节毛状体的发育，并通过影响细胞质 H2O 含量而赋予对蚜虫的抗性。转录组分析揭示了与发病机制、钙结合和纤维素合酶相关的基因显着富集。总的来说，我们的研究阐明了一种改变多细胞毛状体形态并增强对蚜虫抗性的未知机制，从而为黄瓜毛状体形态发生提供了全新的视角。