Understanding the intricate network of biomolecular interactions that govern cellular processes is a fundamental pursuit in biology. Over the past decade, photocatalytic proximity labeling has emerged as one of the most powerful and versatile techniques for studying these interactions as well as uncovering subcellular trafficking patterns, drug mechanisms of action, and basic cellular physiology. In this article, we review the basic principles, methodologies, and applications of photocatalytic proximity labeling as well as examine its modern development into currently available platforms. We also discuss recent key studies that have successfully leveraged these technologies and importantly highlight current challenges faced by the field. Together, this review seeks to underscore the potential of photocatalysis in proximity labeling for enhancing our understanding of cell biology while also providing perspective on technological advances needed for future discovery.

了解控制细胞过程的生物分子相互作用的复杂网络是生物学的基本追求。在过去的十年中，光催化邻近标记已成为研究这些相互作用以及揭示亚细胞运输模式、药物作用机制和基本细胞生理学的最强大和通用的技术之一。在本文中，我们回顾了光催化邻近标记的基本原理、方法和应用，并研究了其在现有平台上的现代发展。我们还讨论了最近成功利用这些技术的关键研究，并重点强调了该领域当前面临的挑战。总之，这篇综述旨在强调光催化在邻近标记中的潜力，以增强我们对细胞生物学的理解，同时也提供未来发现所需的技术进步的观点。