The spontaneous healing of critical-sized bone defects is often limited, posing an increased risk of complications and suboptimal outcomes. Osteogenesis, a complex process central to bone formation, relies significantly on the pivotal role of osteoblasts. Despite the well-established osteogenic properties of vitamin D (VD), its lipophilic nature confines administration to oral or muscle injection routes. Therefore, a strategic therapeutic approach involves designing a multifunctional carrier to enhance efficacy, potentially incorporating it into the delivery system. Here, we introduce an innovative sterosome-based delivery system, utilizing palmitic acid (PA) and VD, aimed at promoting osteogenic differentiation and facilitating post-defect bone regeneration. The delivery system exhibited robust physical characteristics, including excellent stability, loading efficiency, sustained drug release and high cellular uptake efficiency. Furthermore, comprehensive investigations demonstrated outstanding biocompatibility and osteogenic potential in both 2D and 3D settings. A critical-sized calvarial defect model in mice recapitulated the notable osteogenic effects of the sterosomes . Collectively, our research proposes a clinically applicable strategy for bone healing, leveraging PA/VD sterosomes as an efficient carrier to deliver VD and enhance bone regenerative effects.

中文翻译：

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通过立体体技术高效输送维生素 D3 来增强骨再生的简便而明智的策略


临界大小的骨缺损的自发愈合通常是有限的，导致并发症和次优结果的风险增加。成骨是骨形成的一个复杂过程，很大程度上依赖于成骨细胞的关键作用。尽管维生素 D (VD) 具有公认的成骨特性，但其亲脂性限制了口服或肌肉注射途径的给药。因此，战略性治疗方法涉及设计多功能载体以增强功效，并可能将其纳入递送系统。在这里，我们介绍了一种基于甾体的创新递送系统，利用棕榈酸（PA）和VD，旨在促进成骨分化并促进缺损后骨再生。该递送系统表现出强大的物理特性，包括优异的稳定性、装载效率、持续的药物释放和高细胞摄取效率。此外，全面的研究表明在 2D 和 3D 环境中具有出色的生物相容性和成骨潜力。小鼠临界尺寸颅骨缺损模型再现了甾体的显着成骨作用。总的来说，我们的研究提出了一种临床适用的骨愈合策略，利用 PA/VD 甾体作为有效载体来传递 VD 并增强骨再生效果。