Acinetobacter johnsonii and Shewanella putrefaciens were identified as specific spoilage organisms in aquatic food. The interactions among specific spoilage organisms under cold stress have a significant impact on the assembly of microbial communities, which play crucial roles in the spoilage and cold adaptation processes. The limited understanding of A. johnsonii and S. putrefaciens interactions in the cold adaptation mechanism hinders the elucidation of their roles in protein and metabolism levels. 4D quantitative proteomic analysis showed that the coculture of A. johnsonii and S. putrefaciens responds to low temperatures through ABC transporter proteins, resulting in phospholipid transport and inner membrane components. SapA and FtsX proteins were significantly upregulated, while LolC, LolD, LolE, PotD, PotA, PotB, and PotC proteins were significantly downregulated. Metabolome assays revealed that metabolites of glutathione and spermidine/putrescin were significantly upregulated, while metabolites of arginine/lysine/ornithine were significantly downregulated and involved in the ABC transporter metabolism. The results of ultramicroscopic analyses showed that the coculture of A. johnsonii and S. putrefaciens surface combined with the presence of the leakage of intracellular contents, suggesting that the bacteria were severely damaged and wrinkled to absorb metabolic nutrients and adapt to cold temperatures.

中文翻译：

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约氏不动杆菌和腐败希瓦氏菌的共培养有助于影响水产食品储存环境冷适应的 ABC 转运蛋白

约氏不动杆菌和腐败希瓦氏菌被鉴定为水产食品中特定的腐败微生物。冷胁迫下特定腐败生物之间的相互作用对微生物群落的组装产生重大影响，而微生物群落在腐败和冷适应过程中发挥着至关重要的作用。对约氏放线菌和腐败链球菌在冷适应机制中相互作用的了解有限，阻碍了阐明它们在蛋白质和代谢水平中的作用。 4D 定量蛋白质组分析表明，约氏放线菌和腐败链球菌的共培养物通过 ABC 转运蛋白对低温做出反应，从而导致磷脂转运和内膜成分。 SapA和FtsX蛋白显着上调，而LolC、LolD、LolE、PotD、PotA、PotB和PotC蛋白显着下调。代谢组分析显示，谷胱甘肽和亚精胺/腐胺的代谢物显着上调，而精氨酸/赖氨酸/鸟氨酸的代谢物显着下调并参与ABC转运蛋白代谢。超显微分析结果表明，共培养的约氏放线菌和腐败链球菌表面结合细胞内内容物渗漏的存在，表明细菌在吸收代谢养分和适应寒冷温度时受到严重破坏和皱缩。