We design a p-aminothiophenol (pATP) modified Au/ITO chip to determine nitrite ions in lake water by a ratiometric surface-enhanced Raman scattering (SERS) method based on nitrite ions triggering the transformation of pATP to p,p′-dimercaptoazobenzene (DMAB). Intriguingly, by using the SERS peak (at 1008 cm⁻¹) from benzoic ring deforming as an internal standard instead of the traditional peak at 1080 cm⁻¹, the detection sensitivity of the method was improved 10 times.

中文翻译：

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内标优化提高了比率检测方法的灵敏度和稳健性

我们设计了一种对氨基苯硫酚 (pATP) 修饰的 Au/ITO 芯片，通过基于亚硝酸盐离子触发 pATP 转化为对，对'-二巯基偶氮苯的比率表面增强拉曼散射 (SERS) 方法来测定湖水中的亚硝酸盐离子。二甲氨基苯甲酸）。有趣的是，通过使用苯甲酸环变形的SERS峰（1008 cm ^-1）作为内标代替传统的1080 cm ^-1峰，该方法的检测灵敏度提高了10倍。