The cost-effective treatment of sludge leachate (SL) with high nitrogen content and refractory dissolved organic matter (rDOM) has drawn increasing attention. This study employed, for the first time, a rDOM triggered denitratation-anammox continuous-flow process to treat landfill SL. Moreover, the mechanisms of exploiting rDOM from SL as an inner carbon source for denitratation were systematically analyzed. The results demonstrated outstanding nitrogen and rDOM removal performance without any external carbon source supplement. In this study, effluent concentrations of 4.27 ± 0.45 mgTIN/L and 5.58 ± 1.64 mgTN/L were achieved, coupled with an impressive COD removal rate of 65.17 % ± 1.71 %. The abundance of bacteria belonging to the genus, which were identified as rDOM degradation bacteria, increased from 18.23 % to 35.62 %. As a result, various types of rDOM were utilized to different extents, with proteins being the most notable, except for lignins. Metagenomic analysis revealed a preference for directing electrons towards NO-N reductase rather than NO-N reductase, indicating the coupling of denitratation bacteria and anammox bacteria (). Overall, this study introduced a novel synergy platform for advanced nitrogen removal in treating SL using its inner carbon source. This approach is characterized by low energy consumption and operational costs, coupled with commendable efficiency.

中文翻译：

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污泥渗滤液中难降解有机物触发厌氧氨氧化与反硝化结合，实现深度脱氮


高氮含量污泥渗滤液（SL）和难降解有机物（rDOM）的经济有效处理越来越受到关注。本研究首次采用rDOM触发脱硝-厌氧氨氧化连续流工艺来处理垃圾填埋场SL。此外，系统分析了利用SL中的rDOM作为脱硝内碳源的机制。结果表明，无需任何外部碳源补充即可实现出色的氮和 rDOM 去除性能。在这项研究中，出水浓度达到了 4.27 ± 0.45 mgTIN/L 和 5.58 ± 1.64 mgTN/L，COD 去除率高达 65.17 % ± 1.71 %。该属细菌，被鉴定为rDOM降解菌，丰度从18.23%增加到35.62%。因此，各种类型的 rDOM 都得到了不同程度的利用，其中蛋白质最为引人注目，木质素除外。宏基因组分析揭示了电子倾向于导向 NO-N 还原酶而不是 NO-N 还原酶，这表明脱硝细菌和厌氧氨氧化细菌之间存在耦合 ()。总体而言，本研究引入了一种新型协同平台，用于利用其内部碳源处理 SL 进行高级脱氮。这种方法的特点是能耗和运营成本低，而且效率值得称赞。