tions that support specific physiologic processes. However, the heterogeneity of human kidney endothelial cells has not yet been systematically described. Methods We reprocessed and integrated seven human kidney control single-cell/single-nucleus RNA sequencing datasets of >200,000 kidney cells in the same process. Results We identified five major cell types, 29,992 of which were endothelial cells. Endothelial cell reclustering identified seven subgroups that differed in molecular characteristics and physiologic functions. Mapping new data to a normal kidney endothelial cell atlas allows rapid data annotation and analysis. We confirmed that endothelial cell types in the human kidney were also highly conserved in the mouse kidney and identified endothelial marker genes that were conserved in humans and mice, as well as differentially expressed genes between corresponding subpopulations. Furthermore, combined analysis of single-cell transcriptome data with public genome-wide association study data showed a significant enrichment of endothelial cells, especially arterial endothelial cells, in BP heritability. Finally, we identified M1 and M12 from coexpression networks in endothelial cells that may be deeply involved in BP regulation. Conclusions We created a comprehensive reference atlas of normal human kidney endothelial cells that provides the molecular foundation for understanding how the identity and function of kidney endothelial cells are altered in disease, aging, and between species. Finally, we provide a publicly accessible online tool to explore the datasets described in this work (https://vascularmap.jiangnan.edu.cn)....

中文翻译：

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人肾内皮细胞的综合单细胞转录组图谱

支持特定生理过程的因素。然而，人肾内皮细胞的异质性尚未得到系统描述。方法 我们在同一过程中重新处理并整合了超过 200,000 个肾细胞的 7 个人类肾脏对照单细胞/单核 RNA 测序数据集。结果 我们鉴定了五种主要细胞类型，其中 29,992 个是内皮细胞。内皮细胞重新聚集识别出分子特征和生理功能不同的七个亚群。将新数据映射到正常肾内皮细胞图谱可以实现快速数据注释和分析。我们证实，人类肾脏的内皮细胞类型在小鼠肾脏中也高度保守，并鉴定了人类和小鼠中保守的内皮标记基因，以及相应亚群之间的差异表达基因。此外，单细胞转录组数据与公共全基因组关联研究数据的综合分析显示，内皮细胞，尤其是动脉内皮细胞的血压遗传力显着富集。最后，我们从内皮细胞的共表达网络中鉴定出可能深度参与血压调节的M1和M12。结论 我们创建了正常人肾内皮细胞的综合参考图谱，为理解肾内皮细胞的身份和功能如何在疾病、衰老和物种间改变提供了分子基础。最后，我们提供了一个可公开访问的在线工具来探索本工作中描述的数据集（https://angiomap.jiannan.edu.cn）......