Herein, we proposed a novel bioenzyme-free dual-mode sensing strategy for the rapid and accurate monitoring of organophosphorus pesticides (OPs) by preparing tris(2,2′-bipyridyl) ruthenium(II) (Ru(bpy)) encapsulated with UiO-66-NH MOFs as nanozyme (Ru@Zr-MOFs). The as-prepared Ru@Zr-MOFs nanozyme not only exhibits superior peroxidase-like activity which can catalyze O-phenylenediamine (OPD) oxidation to generate yellow 2,3-diaminophenazinc, but also exhibits dual-fluorescence feature (at 442 and 612 nm respectively) and specific interaction capacity with OPs. However, in the existence of OPs, the peroxidase-like activity of Ru@Zr-MOFs nanozyme is restricted and the oxidation process of OPD is stopped; meanwhile, the fluorescence of Ru@Zr-MOFs at 442 nm increases rapidly (< 10 s) while that at 612 nm is apathetic, thus a colorimetric and ratiometric fluorescent sensing strategy was first designed to detect OPs by using glyphosate (Gly) as a typical model of OPs. Then, in consideration of the benefits of smartphone which can make the sensing process more convenient, stable, rapid and sensitive feedback, a smartphone coupled with hydrogel detection platform was proposed also for OPs determination. In addition, the designed sensing strategy offers superior selectivity, stability and real application capabilities. It’s thus expected the as-designed Ru@Zr-MOFs nanozyme based dual-mode sensing strategy possesses important real application for the low-cost, rapid, sensitive and accurate determination of OPs.

中文翻译：

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基于UiO-66-NH2 MOFs封装三(2,2′-联吡啶)钌(II)的有机磷农药无生物酶双模式传感

在此，我们提出了一种新型无生物酶双模式传感策略，通过制备UiO封装的三(2,2'-联吡啶)钌(II) (Ru(bpy))来快速准确地监测有机磷农药(OPs) -66-NH MOFs 作为纳米酶 (Ru@Zr-MOFs)。所制备的Ru@Zr-MOFs纳米酶不仅表现出优异的类过氧化物酶活性，可以催化邻苯二胺（OPD）氧化生成黄色2,3-二氨基吩嗪，而且还表现出双荧光特征（442和612 nm）分别）和与OP的特定相互作用能力。然而，在OPs的存在下，Ru@Zr-MOFs纳米酶的类过氧化物酶活性受到限制，OPD的氧化过程被停止；同时，Ru@Zr-MOFs在442 nm处的荧光快速增加（< 10 s），而在612 nm处的荧光却很迟钝，因此首先设计了一种比色法和比率法荧光传感策略，以草甘膦（Gly）为检测剂来检测OPs。 OP 的典型模型。然后，考虑到智能手机可以使传感过程更加方便、稳定、快速和灵敏反馈的优点，提出了一种智能手机结合水凝胶检测平台也用于OPs测定。此外，所设计的传感策略提供了卓越的选择性、稳定性和实际应用能力。因此，预计所设计的基于 Ru@Zr-MOFs 纳米酶的双模式传感策略对于低成本、快速、灵敏和准确的 OP 测定具有重要的实际应用。