In this study, for the first time, the erbium-doped graphene quantum dots (Er-GQDs) were decorated onto a three-dimensional MoS nanoflower (EGM) for electrochemically sensitive and selective detection of carbohydrate antigen, CA 19-9, in phosphate buffer saline (PBS) and biological fluids. The MoS nanoflowers (MoS NF) were hydrothermally prepared at 200 °C for 24 h, and then 2–8 nm of Er-GQDs were uniformly deposited onto 200 nm MoS NF. The MoS NF has a high electric conductivity for electrochemical performance, whereas Er-GQDs act as an effective anchor with anti-CA19-9 antibodies (CA19-9 Ab) for ultra-selectivity to CA19-9 antigen. The SPE||EGM/Ab immunosensor has substantial benefits by providing a long-lasting, ultra-sensitive, and highly selective platform for CA19-9 antigen detection. The dynamic ranges in PBS, human serum, and saliva are 5 × 10–250, 5 × 10–250, and 3 × 10–250 U mL, respectively, with limits of detection (LODs) of (0.18–2.95) × 10 U mL. Furthermore, the SPE||EGM/Ab immunosensor can detect CA19-9 in human serum with a recovery of 97–108 %. The non-invasive detection of CA19-9 in saliva is stably sensitive before and after 1 h of breakfast. Moreover, the SPE||EGM/Ab immunosensor has excellent selectivity toward CA19-9 over the other 17 interferences. Results elaborate that the SPE||EGM/Ab immunosensor is a promising electrochemical biosensing probe for detecting CA19-9 in human serum and saliva for clinical diagnosis and prognosis of pancreatic cancer in the early stage.

中文翻译：

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使用 Er-GQDs 修饰的 MoS2 纳米花进行胰腺癌快速诊断和早期检测的电化学传感器

在这项研究中，首次将掺铒石墨烯量子点（Er-GQD）装饰到三维MoS纳米花（EGM）上，用于电化学敏感和选择性检测磷酸盐中的碳水化合物抗原CA 19-9缓冲盐水 (PBS) 和生物液体。 MoS2纳米花（MoS2 NF）在200℃下水热24小时制备，然后2-8nm的Er-GQD均匀沉积在200nm MoS2NF上。 MoS NF 具有高电导率，可实现电化学性能，而 Er-GQD 可作为抗 CA19-9 抗体 (CA19-9 Ab) 的有效锚定物，对 CA19-9 抗原具有超选择性。 SPE||EGM/Ab 免疫传感器为 CA19-9 抗原检测提供持久、超灵敏和高选择性的平台，具有显着的优势。 PBS、人血清和唾液的动态范围分别为 5 × 10–250、5 × 10–250 和 3 × 10–250 U mL，检测限 (LOD) 为 (0.18–2.95) × 10单位毫升。此外，SPE||EGM/Ab 免疫传感器可以检测人血清中的 CA19-9，回收率为 97-108%。早餐前后1 h唾液中CA19-9的无创检测灵敏度稳定。此外，SPE||EGM/Ab 免疫传感器对 CA19-9 比其他 17 种干扰物具有优异的选择性。结果表明，SPE||EGM/Ab 免疫传感器是一种很有前途的电化学生物传感探针，用于检测人血清和唾液中的 CA19-9，用于胰腺癌的早期临床诊断和预后。