Enteric virus concentration in large-volume water samples is crucial for detection and essential for assessing water safety. Certain dissolution and suspension components can affect the enrichment process. In this study, tangential flow ultrafiltration (TFUF) was used as an enrichment method for recovering enteric virus in water samples. Interestingly, the bacteriophage MS2 recovery in reclaimed water and the reclaimed water without particles were higher than that in ultrapure water. The simulated reclaimed water experiments showed that humic acid (HA) (92.16% ± 4.32%) and tryptophan (Try) (81.50 ± 7.71%) enhanced MS2 recovery, while the presence of kaolin (Kaolin) inhibited MS2 recovery with an efficiency of 63.13% ± 11.17%. Furthermore, Atomic force microscopy (AFM) revealed that the MS2-HA cluster and the MS2-Try cluster had larger roughness values on the membrane surface, making it difficult to be eluted, whereas MS2-Kaolin cluster had compact surfaces making it difficult to be eluted. Additionally, the MS2-HA cluster is bound to the membrane by single hydrogen bond with SO, whereas both the MS2-Try cluster and the MS2-Kaolin cluster are bound to the membrane by two hydrogen bonds, making eluting MS2 challenging. These findings have potential implications for validating standardized methods for virus enrichment in water samples.

中文翻译：

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悬浮颗粒和溶解有机物对两步切向流超滤中水病毒富集的影响：现象与机制


大量水样中肠道病毒的浓度对于检测至关重要，对于评估水安全也至关重要。某些溶解和悬浮成分会影响浓缩过程。在本研究中，切向流超滤（TFUF）被用作回收水样中肠道病毒的富集方法。有趣的是，中水和无颗粒中水的噬菌体MS2回收率均高于超纯水。模拟再生水实验表明，腐殖酸（HA）（92.16%±4.32%）和色氨酸（Try）（81.50±7.71%）增强了MS2回收率，而高岭土（Kaolin）的存在抑制了MS2回收率，效率为63.13 % ± 11.17%。此外，原子力显微镜（AFM）显示，MS2-HA团簇和MS2-Try团簇膜表面粗糙度值较大，难以洗脱，而MS2-Kaolin团簇表面致密，难以被洗脱。洗脱。此外，MS2-HA 簇通过与 SO 的单氢键与膜结合，而 MS2-Try 簇和 MS2-高岭土簇均通过两个氢键与膜结合，这使得洗脱 MS2 具有挑战性。这些发现对于验证水样中病毒富集的标准化方法具有潜在意义。