The fungus is a thermotolerant microorganism that has been explored as a reservoir for enzymes (hydrolytic enzymes and oxidoreductases). The functional analysis of a recombinant cellobiose dehydrogenase (MtCDHB) from demonstrated a thermophilic behavior, an optimal pH in alkaline conditions for inter-domain electron transfer, and catalytic activity on cellooligosaccharides with different degree of polymerization. Its applicability was evaluated to the sustainable production of cellobionic acid (CBA), a potential pharmaceutical and cosmetic ingredient rarely commercialized. Dissolving pulp was used as a disaccharide source for MtCDHB. Initially, recombinant exoglucanases (MtCBHI and MtCBHII) from hydrolyzed the dissolving pulp, resulting in 87% cellobiose yield, which was subsequently converted into CBA by MtCDHB, achieving a 66% CBA yield after 24 h. These findings highlight the potential of MtCDHB as a novel approach to obtaining CBA through the bioconversion of a plant-based source.

中文翻译：

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来自嗜热嗜热酵母的重组纤维二糖脱氢酶：其功能表征及其在纤维二酸生产中的适用性


真菌是一种耐热微生物，已被探索作为酶（水解酶和氧化还原酶）的储存库。重组纤维二糖脱氢酶 (MtCDHB) 的功能分析证明了其嗜热行为、碱性条件下域间电子转移的最佳 pH 值以及对不同聚合度纤维寡糖的催化活性。其在纤维二酸（CBA）的可持续生产中的适用性进行了评估，纤维二酸是一种很少商业化的潜在药物和化妆品成分。溶解浆用作 MtCDHB 的二糖源。最初，重组外切葡聚糖酶（MtCBHI 和 MtCBHII）水解溶解浆，产生 87% 的纤维二糖产量，随后通过 MtCDHB 将其转化为 CBA，24 小时后实现 66% 的 CBA 产量。这些发现凸显了 MtCDHB 作为一种通过植物来源的生物转化获得 CBA 的新方法的潜力。