Since the outbreak of monkeypox (mpox) in 2022, widespread concern has been placed on imposing an urgent demand for specific vaccines that offer safer and more effective protection. Using an efficient and scalable circular RNA (circRNA) platform, we constructed four circRNA vaccines that could induce robust neutralizing antibodies as well as T cell responses by expressing different surface proteins of mpox virus (MPXV), resulting in potent protection against vaccinia virus (VACV) in mice. Strikingly, the combination of the four circular RNA vaccines demonstrated the best protection against VACV challenge among all the tested vaccines. Our study provides a favorable approach for developing MPXV-specific vaccines by using a circular mRNA platform and opens up novel avenues for future vaccine research.

中文翻译：

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抗猴痘病毒的环状 RNA 疫苗可有效保护小鼠免受痘苗病毒感染


自 2022 年猴痘 (mpox) 爆发以来，人们普遍关注对提供更安全、更有效保护的特定疫苗的迫切需求。使用高效且可扩展的环状 RNA (circRNA) 平台，我们构建了四种 circRNA 疫苗，它们可以通过表达痘苗病毒 (MPXV) 的不同表面蛋白来诱导强大的中和抗体以及 T 细胞反应，从而对痘苗病毒 (VACV) 产生有效的保护）在小鼠中。引人注目的是，在所有测试的疫苗中，四种环状 RNA 疫苗的组合证明了针对 VACV 攻击的最佳保护作用。我们的研究为利用环状 mRNA 平台开发 MPXV 特异性疫苗提供了有利的方法，并为未来的疫苗研究开辟了新的途径。