Pigmentation-related mutations can be utilized to distinguish between differentially colored sectors of chimeric thalli, thereby facilitating the efficient breeding of economically valuable Pyropia/Porphyra seaweed species. However, the specific trait loci and alleles responsible for Pyropia/Porphyra coloration have yet to be identified, which limits the applicability of coloration mutants for breeding and genetic analyses. In this study, to preserve the genetic integrity of the population, only four-colored thalli were considered when constructing the doubled haploid (DH) Pyropia haitanensis population, which consisted of 480 homozygous offspring lines (representing the largest DH Pyropia/Porphyra population). The offspring lines in the DH population exhibited both wild-type colored and orange sectors, with a segregation ratio of approximately 1:1, indicating that the orange coloration was controlled by a single nuclear gene. Through BSA-seq analysis (99% confidence interval), a candidate region of 0.5 Mb was identified in the P. haitanensis genome. Additionally, a non-synonymous SNP [A/G] was detected at base-pair position 481 in the coding region of PhcpcC, which encodes a phycocyanin-associated rod linker protein. This SNP locus was verified in both DH and natural populations, with the wild-type colored lines having an A base and the orange lines having a G base at this locus. Therefore, PhcpcC may be the gene associated with the orange coloration of P. haitanensis. The molecular marker developed in this study can be employed to exploit pigmentation mutants for breeding and genetic analyses of Pyropia/Porphyra species.

中文翻译：

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坛紫菜橙色相关基因PhcpcC的鉴定

色素相关突变可用于区分嵌合菌体的不同颜色部分，从而促进具有经济价值的高效育种紫菜/紫菜海藻种类。然而，负责的特定性状基因座和等位基因紫菜/紫菜着色尚未确定，这限制了着色突变体在育种和遗传分析中的适用性。在本研究中，为了保持群体的遗传完整性，在构建双单倍体（DH）时仅考虑四色菌体坛紫菜群体，由 480 个纯合后代系组成（代表最大的 DH紫菜/紫菜人口）。 DH群体中的子代品系同时表现出野生型颜色和橙色区域，分离比约为1:1，表明橙色颜色由单个核基因控制。通过 BSA-seq 分析（99% 置信区间），在坛松基因组。此外，在编码区的碱基对位置 481 处检测到非同义 SNP [A/G]磷酸氯乙烯，它编码藻蓝蛋白相关的杆连接蛋白。该 SNP 位点在 DH 和自然群体中得到验证，野生型彩色品系在该位点具有 A 碱基，橙色品系具有 G 碱基。所以，磷酸氯乙烯可能是与橙色相关的基因坛松。本研究开发的分子标记可用于开发色素沉着突变体，用于育种和遗传分析紫菜/紫菜物种。