Summary Water plays crucial roles in expeditious growth and osmotic stress of bamboo. Nevertheless, the molecular mechanism of water transport remains unclear. In this study, an aquaporin gene, PeTIP4‐3, was identified through a joint analysis of root pressure and transcriptomic data in moso bamboo (Phyllostachys edulis). PeTIP4‐3 was highly expressed in shoots, especially in the vascular bundle sheath cells. Overexpression of PeTIP4‐3 could increase drought and salt tolerance in transgenic yeast and rice. A co‐expression pattern of PeSAPK4, PeMYB99 and PeTIP4‐3 was revealed by WGCNA. PeMYB99 exhibited an ability to independently bind to and activate PeTIP4‐3, which augmented tolerance to drought and salt stress. PeSAPK4 could interact with and phosphorylate PeMYB99 in vivo and in vitro, wherein they synergistically accelerated PeTIP4‐3 transcription. Overexpression of PeMYB99 and PeSAPK4 also conferred drought and salt tolerance in transgenic rice. Further ABA treatment analysis indicated that PeSAPK4 enhanced water transport in response to stress via ABA signaling. Collectively, an ABA‐mediated cascade of PeSAPK4‐PeMYB99‐PeTIP4‐3 is proposed, which governs water transport in moso bamboo.

中文翻译：

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涉及水运输的竹“PeSAPK4-PeMYB99-PeTIP4-3”调控模型

概括 水在竹子的快速生长和渗透压中起着至关重要的作用。然而，水传输的分子机制仍不清楚。 在这项研究中，水通道蛋白基因，PeTIP4-3，是通过毛竹根压和转录组数据的联合分析确定的（毛竹）。PeTIP4-3在芽中高表达，尤其是在维管束鞘细胞中。过度表达PeTIP4-3可以提高转基因酵母和水稻的耐旱性和耐盐性。 共表达模式PeSAPK4,佩MYB99和PeTIP4-3WGCNA 透露。 PeMYB99表现出独立结合和激活的能力PeTIP4-3，增强了对干旱和盐胁迫的耐受性。 PeSAPK4 可以与 PeMYB99 相互作用并磷酸化 PeMYB99体内和体外，其中它们协同加速PeTIP4-3转录。过度表达佩MYB99和PeSAPK4还赋予转基因水稻耐旱和耐盐性。 进一步的 ABA 处理分析表明，PeSAPK4 通过 ABA 信号传导增强水传输以响应应激。总的来说，ABA 介导的 PeSAPK4-PeMYB99- 级联PeTIP4-3提议，它控制着毛竹的水运输。