BackgroundThe aim of this study is to evaluate the immune regulation and tissue remodeling responses during experimental gingivitis (EG) and naturally occurring gingivitis (NG) to provide a comprehensive analysis of host responses. Gingival crevicular fluid (GCF) was obtained from 2 human studies conducted in university settings.MethodsThe EG study enrolling 26 volunteers provided controls for the baseline (Day 0) from healthy disease‐free participants, while Day 21 (the end of EG induction of the same group) was used to represent EG. Twenty‐six NG participants age‐matched with those of the EG group were recruited. GCF samples were analyzed for 39 mediators of inflammatory/immune responses and tissue remodeling using commercially available bead‐based multiplex immunoassays. The differences in GI and mediator expression among groups were determined at a 95% confidence level (p ≤ 0.05) by a 2‐way analysis of variance (ANOVA) with a post‐hoc Tukey's test.ResultsOur findings showed that EG had a greater gingival index than NG and was healthy (p < 0.01 of all comparisons). Furthermore, EG showed significantly higher levels of MPO (p < 0.001), CCL3 (p < 0.05), and IL‐1B (p < 0.001) than NG. In contrast, NG had increased levels of MIF (p < 0.05), Fractalkine (p < 0.001), angiogenin (p < 0.05), C3a (p < 0.001), BMP‐2 (p < 0.001), OPN (p < 0.05), RANKL (p < 0.001), and MMP‐13 (p < 0.001) than EG.ConclusionsConsistent with the findings from chronic (NG) versus acute (EG) inflammatory lesions, these data reveal that NG displays greater immune regulation, angiogenesis, and bone remodeling compared to EG.

中文翻译：

---

牙龈炎期间炎症和组织重塑介质的表达：实验性、自然发生的牙龈炎与牙周健康之间的比较

背景本研究的目的是评估实验性牙龈炎（EG）和自然发生的牙龈炎（NG）期间的免疫调节和组织重塑反应，以对宿主反应进行全面分析。龈沟液 (GCF) 是从在大学环境中进行的 2 项人体研究中获得的。方法 EG 研究招募了 26 名志愿者，为健康无病参与者的基线（第 0 天）提供对照，而第 21 天（EG 诱导结束）相同的组）被用来代表EG。招募了 26 名与 EG 组年龄匹配的 NG 参与者。使用市售的基于微珠的多重免疫测定法对 GCF 样本中的 39 种炎症/免疫反应和组织重塑介质进行了分析。各组之间 GI 和介质表达的差异以 95% 的置信水平确定（p≤ 0.05），通过事后 Tukey 检验进行双向方差分析 (ANOVA)。结果我们的研究结果表明，EG 的牙龈指数比 NG 更大，并且是健康的（p< 所有比较的 0.01）。此外，EG 显示出显着更高的 MPO 水平（p< 0.001), CCL3 (p< 0.05) 和 IL-1B (p< 0.001) 比 NG。相反，NG 的 MIF 水平增加（p< 0.05), 分形线 (p< 0.001), 血管生成素 (p< 0.05), C3a (p< 0.001), BMP-2 (p< 0.001), OPN (p< 0.05), RANKL (p< 0.001) 和 MMP-13 (p< 0.001) 比 EG。结论与慢性 (NG) 与急性 (EG) 炎症病变的结果一致，这些数据表明，与 EG 相比，NG 显示出更强的免疫调节、血管生成和骨重塑。