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Cytochrome GmGLY1 is Involved in the Biosynthesis of Glycitein in Soybean
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2024-05-06 , DOI: 10.1021/acs.jafc.4c00968
Peipei Zhang 1, 2 , Changyun Yang 1 , Jiao Wang 1 , Pingbo Jiang 1 , Jie Qi 1 , Wenyan Hou 1 , Hao Cheng 1 , Xianzhong Feng 2 , Deyue Yu 1
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Isoflavones, the major secondary metabolites of interest due to their benefits to both human and plant health, are exclusively produced by legumes. In this study, we profiled the isoflavone content in dry seeds from 211 soybean [Glycine max (L.) Merr.] accessions grown across five environments. Broad and discernible phenotypic variations were observed among accessions, regions, and years of growth. Twenty-six single-nucleotide polymorphisms (SNPs) associated with the sum of glycitein (GLE), glycitin (GL), 6″-O-acetylglycitin (AGL), and 6″-O-malonylglycitin (MGL) contents were detected in multiple environments via a genome-wide association study (GWAS). These SNPs were located on chromosome 11 (8,148,438 bp to 8,296,956 bp, renamed qGly11–01). Glyma.11g108300 (GmGLY1), a gene that encodes a P450 family protein, was identified via sequence variation analysis, functional annotation, weighted gene coexpression network analysis (WGCNA), and expression profile analysis of candidate gene, and hairy roots transformation in soybean. Overexpression of GmGLY1 increased the glycitein content (GLC) in soybean hairy roots and transgenic seeds, while CRISPR/Cas9-generated mutants exhibited decreased GLC and increased daidzein content (DAC). Haplotype analysis revealed that GmGLY1 allelic variations significantly affect the GLC accumulation. These findings enhance our understanding of genes influencing GLC in soybean and may guide breeding for lines with high and stable GLC.

中文翻译:

细胞色素 GmGLY1 参与大豆中黄豆黄素的生物合成

异黄酮是主要的次生代谢物,因其对人类和植物健康有益而完全由豆类产生。在这项研究中,我们分析了在五个环境中生长的 211 颗大豆 [ Glycine max (L.) Merr.] 种质干种子中的异黄酮含量。在种质、地区和生长年份之间观察到广泛且可辨别的表型变异。在多个样本中检测到 26 个与黄豆黄素 (GLE)、黄豆黄素 (GL)、6”-O-乙酰黄豆黄素 (AGL) 和 6”-O-丙酰基黄豆黄素 (MGL) 含量总和相关的单核苷酸多态性 (SNP)。通过全基因组关联研究(GWAS)的环境。这些 SNP 位于 11 号染色体上(8,148,438 bp 至 8,296,956 bp,重命名为qGly11–01)。通过序列变异分析、功能注释、加权基因共表达网络分析(WGCNA)、候选基因表达谱分析以及大豆毛状根转化,鉴定出编码P450家族蛋白的基因Glyma.11g108300GmGLY1 )。 GmGLY1的过表达增加了大豆毛状根和转基因种子中的黄豆黄素含量 (GLC),而 CRISPR/Cas9 生成的突变体表现出 GLC 减少和大豆黄素含量 (DAC) 增加。单倍型分析表明,GmGLY1等位基因变异显着影响 GLC 积累。这些发现增强了我们对影响大豆 GLC 的基因的理解,并可能指导培育具有高且稳定 GLC 的品系。
更新日期:2024-05-06
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