In this study, 20-day-old soybean plants were watered with 100 mL of 100 mM NaCl solution and sprayed with silica nanoparticles (SiO₂ NPs) or potassium silicate every 3 days over 15 days, with a final dosage of 12 mg of SiO₂ per plant. We assessed the alterations in the plant’s growth and physiological traits, and the responses of bacterial microbiome within the leaf endosphere, rhizosphere, and root endosphere. The result showed that the type of silicon did not significantly impact most of the plant parameters. However, the bacterial communities within the leaf and root endospheres had a stronger response to SiO₂ NPs treatment, showing enrichment of 24 and 13 microbial taxa, respectively, compared with the silicate treatment, which led to the enrichment of 9 and 8 taxonomic taxa, respectively. The rhizosphere bacterial communities were less sensitive to SiO₂ NPs, enriching only 2 microbial clades, compared to the 8 clades enriched by silicate treatment. Furthermore, SiO₂ NPs treatment enriched beneficial genera, such as Pseudomonas, Bacillus, and Variovorax in the leaf and root endosphere, likely enhancing plant growth and salinity stress resistance. These findings highlight the potential of SiO₂ NPs for foliar application in sustainable farming by enhancing plant-microbe interactions to improve salinity tolerance.

中文翻译：

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比较硅纳米颗粒和传统硅缓解大豆（Glycine max L.）盐度胁迫的潜力：生长和生理特性以及根际/内生细菌群落

在这项研究中，用 100 mL 100 mM NaCl 溶液浇灌 20 天的大豆植株，并在 15 天内每 3 天喷洒二氧化硅纳米粒子 (SiO ₂ NPs) 或硅酸钾，最终剂量为 12 mg SiO每株_{2 个}。我们评估了植物生长和生理特性的变化，以及叶内圈、根际和根内圈内细菌微生物组的反应。结果表明，硅的类型并未对大多数工厂参数产生显着影响。然而，叶和根内球内的细菌群落对SiO ₂ NPs处理有更强的响应，与硅酸盐处理相比，分别富集了24和13个微生物类群，从而富集了9和8个分类群。分别。根际细菌群落对SiO ₂ NPs不太敏感，仅富集2个微生物分支，而硅酸盐处理富集了8个分支。此外，SiO ₂ NPs处理丰富了叶和根内圈中的有益属，例如假单胞菌属、芽孢杆菌属和Variovorax属，可能增强植物生长和盐胁迫抵抗力。这些发现凸显了 SiO ₂ NP 通过增强植物-微生物相互作用来提高耐盐性，在可持续农业中叶面喷施的潜力。