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Ultraphotostable Phosphorescent Nanosensors for Sensing the Lysosomal pH at the Single-Cell Level over Long Durations
Analytical Chemistry ( IF 7.4 ) Pub Date : 2024-05-08 , DOI: 10.1021/acs.analchem.4c00696
Zhenzhen Lin 1 , Yinglu Zhang 1 , Longjiang Ding 1 , Xu-dong Wang 1
Affiliation  

Ultraphotostable phosphorescent nanosensors have been designed for continuously sensing the lysosome pH over a long duration. The nanosensors exhibited excellent photostability, high accuracy, and capability to measure pH values during cell proliferation for up to 7 days. By arranging a metal–ligand complex of long phosphorescence lifetime and pH indicator in silica nanoparticles, we discover efficient Förster resonance energy transfer, which converts the pH-responsive UV–vis absorption signal of the pH indicator into a phosphorescent signal. Both the phosphorescent intensity and lifetime change at different pH values, and intracellular pH values can be accurately measured by our custom-built rapid phosphorescent lifetime imaging microscopy. The excellent photostability, high accuracy, and good biocompatibility prove that these nanosensors are a useful tool for tracing the fluctuation of pH values during endocytosis. The methodology can be easily adapted to design new nanosensors with different pKa or for different kinds of intracellular ions, as there are hundreds of pH and ion indicators readily available.

中文翻译:


超光稳定磷光纳米传感器,用于在单细胞水平上长时间感测溶酶体 pH 值



超光稳定磷光纳米传感器设计用于长时间连续检测溶酶体 pH 值。纳米传感器表现出优异的光稳定性、高精度,并且能够在细胞增殖过程中测量 pH 值长达 7 天。通过在二氧化硅纳米粒子中排列长磷光寿命的金属配体络合物和pH指示剂,我们发现了有效的福斯特共振能量转移,它将pH指示剂的pH响应紫外可见吸收信号转换为磷光信号。我们定制的快速磷光寿命成像显微镜可以准确测量不同 pH 值下的磷光强度和寿命变化,以及细胞内 pH 值。优异的光稳定性、高精度和良好的生物相容性证明这些纳米传感器是追踪胞吞过程中pH值波动的有用工具。该方法可以很容易地适应设计具有不同 pK a 或不同种类细胞内离子的新型纳米传感器,因为有数百种 pH 和离子指示剂可供使用。
更新日期:2024-05-08
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