Bacterial adhesion is a serious problem in the healthcare and food industries, causing health problems and economic losses. This study evaluates the generation (LIPSS) with different periodicities to reduce bacterial adhesion on ferritic stainless steels. LIPSS nanostructuring evolves from hydrophilic to hydrophobic with air exposure. Fresh samples with 870 nm LIPSS show nearly 50 % reduced adhesion, but this effect diminishes in stabilized samples. This effect on bacterial adhesion can be attributed to LIPSS periodicity being like bacterial size, which would allow bacteria to accommodate and provide a suitable environment for their adhesion. Our results show that, in stable samples when the LIPSS periodicity is around 400 nm, reductions by almost 30 % are achieved and this reduction reaches 35 % when periodicity is close to 270 nm. These results indicate that smaller spatial periods prevent bacteria from fitting into valleys, so they remain on the peaks, hindering bacterial adhesion. Additionally, deeper LIPSS produced by a higher (SDAE), enhance bacteria-material interaction and, thus, increases adhesion.

中文翻译：

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尺寸很重要：LIPSS 的周期性和深度如何影响大肠杆菌在铁素体不锈钢上的粘附


细菌粘附是医疗保健和食品行业的一个严重问题，会导致健康问题和经济损失。本研究评估了不同周期的生成（LIPSS），以减少铁素体不锈钢上的细菌粘附。随着暴露在空气中，LIPSS 纳米结构从亲水性演变为疏水性。使用 870 nm LIPSS 的新鲜样品显示粘附力降低了近 50%，但这种影响在稳定样品中会减弱。这种对细菌粘附的影响可归因于 LIPSS 周期性，就像细菌的大小一样，这将使细菌能够适应并为其粘附提供合适的环境。我们的结果表明，在稳定样品中，当 LIPSS 周期约为 400 nm 时，减少了近 30%，当周期接近 270 nm 时，减少量达到 35%。这些结果表明，较小的空间周期阻止细菌适应山谷，因此它们保留在山峰上，阻碍细菌粘附。此外，更高的 (SDAE) 产生的更深的 LIPSS 增强了细菌与材料的相互作用，从而增加了粘附力。