In this work, a microfluidic immunosensor chip was developed by incorporating microfluidic technology with electrochemiluminescence (ECL) for sensitive detection of human epidermal growth factor receptor-2 (HER2). The immunosensor chip can achieve robust reproducibility in mass production by integrating multiple detection units in a series. Notably, nanoscale materials can be better adapted to microfluidic systems, greatly enhancing the accuracy of the immunosensor chip. Ag@Au NCs closed by glutathione (GSH) were introduced in the ECL microfluidic immunosensor system with excellent and stable ECL performance. The synthesized CeO₂–Au was applied as a coreaction promoter in the ECL signal amplification system, which made the result of HER2 detection more reliable. In addition, the designed microfluidic immunosensor chip integrated the biosensing system into a microchip, realizing rapid and accurate detection of HER2 by its high throughput and low usage. The developed short peptide ligand NARKFKG (NRK) achieved an effective connection between the antibody and nanocarrier for improving the detection efficiency of the sensor. The immunosensor chip had better storage stability and sensitivity than traditional detection methods, with a wide detection range from 10 fg·mL^–1 to 100 ng·mL^–1 and a low detection limit (LOD) of 3.29 fg·mL^–1. In general, a microfluidic immunosensor platform was successfully constructed, providing a new idea for breast cancer (BC) clinical detection.

中文翻译：

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基于双金属纳米团簇增强型阴极电化学发光灵敏检测人表皮生长因子受体2的微流控免疫传感器平台


本工作将微流控技术与电化学发光（ECL）相结合，开发了一种微流控免疫传感器芯片，用于灵敏检测人表皮生长因子受体2（HER2）。该免疫传感器芯片通过串联集成多个检测单元，可以在批量生产中实现强大的重现性。值得注意的是，纳米级材料可以更好地适应微流控系统，大大提高免疫传感器芯片的准确性。将谷胱甘肽（GSH）封闭的Ag@Au NCs引入ECL微流控免疫传感器系统中，具有优异且稳定的ECL性能。合成的CeO ₂ -Au作为共反应启动子应用于ECL信号放大系统中，使得HER2检测结果更加可靠。此外，设计的微流控免疫传感器芯片将生物传感系统集成到微芯片中，以其高通量和低用量实现了HER2的快速、准确检测。开发的短肽配体NARKFKG（NRK）实现了抗体与纳米载体之间的有效连接，提高了传感器的检测效率。该免疫传感器芯片比传统检测方法具有更好的储存稳定性和灵敏度，检测范围宽，从10 fg·mL ^–1 到100 ng·mL ^–1 ，检测限低（LOD）。 ) 3.29 fg·mL ^–1 。总体而言，成功构建了微流控免疫传感器平台，为乳腺癌（BC）临床检测提供了新思路。