Pressure and temperature, as common physical parameters, are important for monitoring human health. In contrast, single-mode monitoring is prone to causing experimental errors. Herein, we innovatively designed a dual-mode flexible sensing platform based on a platinum/zinc-meso-tetrakis(4-carboxyphenyl)porphyrin (Pt/Zn-TCPP) nanozyme for the quantitative monitoring of carcinoembryonic antigen (CEA) in biological fluids with pressure and temperature readouts. The Pt/Zn-TCPP nanozyme with catalytic and photothermal efficiencies was synthesized by means of integrating photosensitizers into porous materials. The flexible sensing system after the antigen–antibody reaction recognized the pressure using a flexible skin-like pressure sensor with a digital multimeter readout, whereas the temperature was acquired via the photoheat conversion system of the Pt/Zn-TCPP nanozyme under 808 nm near-infrared (NIR) irradiation using a portable NIR imaging camera on a smartphone. Meanwhile, the dual-mode flexible sensing system was carried out on a homemade three-dimensional (3D)-printed device. Results revealed that the developed dual-mode immunosensing platform could exhibit good pressure and temperature responses within the dynamic range of 0.5–100 ng mL^–1 CEA with the detection limits of 0.24 and 0.13 ng mL^–1, respectively. In addition, the pressure and temperature were sensed simultaneously without crosstalk interference. Importantly, the dual-mode flexible immunosensing system can effectively avoid false alarms during the measurement, thus providing great potential for simple and low-cost development for point-of-care testing.

中文翻译：

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基于 Pt/Zn-TCPP 纳米酶的灵活免疫分析，用于低丰度蛋白质的双模式压力-温度监测


压力和温度作为常见的物理参数，对于监测人体健康具有重要意义。相比之下，单模式监测容易造成实验误差。在此，我们创新性地设计了一种基于铂/锌-内消旋-四（4-羧基苯基）卟啉（Pt/Zn-TCPP）纳米酶的双模式柔性传感平台，用于定量监测生物体液中的癌胚抗原（CEA）。压力和温度读数。通过将光敏剂集成到多孔材料中，合成了具有催化和光热效率的Pt/Zn-TCPP纳米酶。抗原抗体反应后的柔性传感系统使用具有数字万用表读数的柔性类皮肤压力传感器识别压力，而温度则通过 Pt/Zn-TCPP 纳米酶在 808 nm 近红外光下的光热转换系统获取。使用智能手机上的便携式 NIR 成像相机进行红外 (NIR) 照射。同时，双模柔性传感系统在自制的三维（3D）打印设备上进行。结果表明，所开发的双模式免疫传感平台在0.5-100 ng mL ^–1 CEA的动态范围内表现出良好的压力和温度响应，检测限为0.24和0.13 ng mL ^–1