Development of reliable methods for the detection of potential biomarkers is of the utmost importance for an early diagnosis of critical diseases and disorders. In this study, a novel lanthanide-functionalized carbon dot-based fluorescent probe Zn-CD@Eu is reported for the ratiometric detection of dipicolinic acid (DPA) and uric acid (UA). The Zn-CD@Eu nanoprobe was obtained from a simple room-temperature reaction of zinc-doped carbon dots (Zn-CD) and the EDTA–Eu lanthanide complex. Under optimal conditions, a good linear response was obtained for DPA in two concentration ranges of 0−55 and 55−100 μM with a limit of detection of 0.53 and 2.2 μM respectively, which is significantly below the infectious dosage of anthrax (∼55 μM). Furthermore, the Zn-CD@Eu/DPA system was employed for the detection of UA with a detection limit of 0.36 μM in the linear range of 0–100 μM. The fluorescent probe was successfully implemented for determining DPA and UA in human blood serum, sweat, and natural water bodies with considerable recovery rates. In addition, the potential of the nanoprobe for ex vivo visualization of UA was demonstrated in fruit fly (Drosophila melanogaster) as a model organism.

中文翻译：

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用于检测吡啶二羧酸、尿酸和离体尿酸成像的 Zn-CD@Eu 比率荧光探针


开发用于检测潜在生物标志物的可靠方法对于危重疾病和病症的早期诊断至关重要。在这项研究中，报道了一种新型的镧系元素功能化碳点荧光探针Zn-CD@Eu，用于吡啶二羧酸（DPA）和尿酸（UA）的比例检测。 Zn-CD@Eu 纳米探针是通过锌掺杂碳点 (Zn-CD) 和 EDTA-Eu 稀土配合物的简单室温反应获得的。在最佳条件下，DPA 在 0−55 和 55−100 μM 两个浓度范围内均获得良好的线性响应，检测限分别为 0.53 和 2.2 μM，显着低于炭疽的感染剂量（~55 μM）。 ）。此外，Zn-CD@Eu/DPA系统用于检测UA，在0-100 μM的线性范围内检测限为0.36 μM。该荧光探针已成功用于测定人体血清、汗液和天然水体中的 DPA 和 UA，且回收率相当高。此外，纳米探针在果蝇（Drosophila melanogaster）作为模型生物体中展示了 UA 离体可视化的潜力。