Benzotriazoles (BTRs) are a class of benzoheterocyclic chemicals that are frequently used as metal-corrosive inhibitors, both in industry and daily use. However, the exposure effect information on BTRs remains relatively limited. In this study, an integrated metabolomic and transcriptomic approach was utilized to evaluate the effect of three BTRs, benzotriazole, 6-chloro-1-hydroxi-benzotriazole, and 1-hydroxy-benzotriazole, in the mouse liver with results showing disrupted basal metabolic processes and vitamin and cofactor metabolism after 28 days. The expression of several genes that are related to the inflammatory response and aryl hydrocarbon receptor pathways, such as Gstt2 and Arntl, was altered by the exposure to BTRs. Exposure to BTRs also affected metabolites and genes that are involved in the immune system and xenobiotic responses. The altered expression of several cytochrome P450 family genes reveal a potential detoxification mechanism in the mouse liver. Taken together, our findings provide new insights into the multilayer response of the mouse liver to BTRs exposure as well as a resource for further exploration of the molecular mechanisms by which the response occurs.

中文翻译：

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使用综合代谢组学和转录组学分析深入了解苯并三唑对肝脏的影响


苯并三唑 (BTR) 是一类苯并杂环化学品，在工业和日常使用中经常用作金属腐蚀抑制剂。然而，BTR 的曝光效果信息仍然相对有限。在这项研究中，采用综合代谢组学和转录组学方法来评估三种 BTR（苯并三唑、6-氯-1-羟基苯并三唑和 1-羟基苯并三唑）在小鼠肝脏中的作用，结果显示基础代谢过程被破坏以及 28 天后的维生素和辅因子代谢。与炎症反应和芳基碳氢化合物受体途径相关的几个基因（例如 Gstt2 和 Arntl）的表达因暴露于 BTR 而发生改变。接触 BTR 还会影响参与免疫系统和外源反应的代谢物和基因。几个细胞色素 P450 家族基因表达的改变揭示了小鼠肝脏中潜在的解毒机制。总而言之，我们的研究结果为小鼠肝脏对 BTR 暴露的多层反应提供了新的见解，并为进一步探索发生反应的分子机制提供了资源。