Background:The vancomycin presoaking technique (wherein grafts are treated with a vancomycin solution [VS] for anterior cruciate ligament reconstruction [ACLR]) reduces the infection rate after ACLR. However, the effects of this technique on graft-bone healing have not been fully elucidated.Purpose:To investigate the effects of vancomycin presoaking on graft-bone healing in a rat ACLR model.Study Design:Controlled laboratory study.Methods:Long flexor digitorum longus tendons were obtained from 9 Wistar rats, and each was randomly allocated to the normal saline (NS) or VS groups. The grafts were immersed in sterile saline for 30 minutes in the NS group and in a 5-mg/mL VS in the VS group. The presence of time-zero graft bacterial contamination was confirmed, and the grafts were incubated in Fluidised Thioglycollate Broth for 2 weeks. ACLR was performed on the right knees of 65 male Wistar rats using the flexor digitorum longus tendons. Each graft was similarly treated. Biomechanical testing, micro–computed tomography, and histological evaluations were performed 4 and 12 weeks postoperatively.Results:The VS group showed significantly reduced graft contamination at time zero ( P = .02). The mean maximum loads to failure were 13.7 ± 8.2 N and 11.6 ± 4.8 N in the NS and VS groups, respectively, at 4 weeks ( P = .95); and 23.2 ± 13.2 N and 30.4 ± 18.0 N in the NS and VS groups, respectively, at 12 weeks ( P = .35). Regarding micro–computed tomography, the mean bone tunnel volumes were 3.76 ± 0.48 mm3 and 4.40 ± 0.58 mm3 in the NS and VS groups, respectively, at 4 weeks ( P = .41); and 3.51 ± 0.38 mm3 and 3.67 ± 0.35 mm3 in the NS and VS groups, respectively, at 12 weeks ( P = .54). Histological semiquantitative examination revealed no clear between-group differences at any time point.Conclusion:Presoaking grafts in vancomycin in a rat ACLR model demonstrated no discernible adverse effects on short- and midterm biomechanical, radiological, and histological investigations.Clinical Relevance:The findings provide guidance for surgeons when considering this technique.

中文翻译：

---

万古霉素预浸泡移植物不会损害大鼠前十字韧带重建模型中的移植物骨愈合

背景：万古霉素预浸泡技术（其中用万古霉素溶液[VS]处理移植物以进行前交叉韧带重建[ACLR]）可降低ACLR后的感染率。然而，该技术对移植骨愈合的影响尚未完全阐明。目的：研究万古霉素预浸泡对大鼠 ACLR 模型移植骨愈合的影响。研究设计：对照实验室研究。方法：长屈指肌取9只Wistar大鼠的长肌腱，将每只随机分配至生理盐水(NS)组或VS组。 NS 组将移植物浸入无菌盐水中 30 分钟，VS 组将移植物浸入 5 mg/mL VS 中。确认了零时间移植物细菌污染的存在，并将移植物在流态化硫代乙醇酸肉汤中孵育 2 周。使用趾长屈肌腱对 65 只雄性 Wistar 大鼠的右膝进行 ACLR。每个移植物均进行类似处理。术后 4 周和 12 周进行生物力学测试、显微计算机断层扫描和组织学评估。结果：VS 组在零时间时显示移植物污染显着减少（P = .02）。 4 周时，NS 组和 VS 组的平均最大失效载荷分别为 13.7 ± 8.2 N 和 11.6 ± 4.8 N ( P = .95)；第 12 周时，NS 组和 VS 组分别为 23.2 ± 13.2 N 和 30.4 ± 18.0 N ( P = .35)。关于显微计算机断层扫描，平均骨隧道体积为 3.76 ± 0.48 mm3和 4.40 ± 0.58 毫米3分别在 NS 组和 VS 组中，第 4 周（P = .41）；和 3.51 ± 0.38 毫米3和 3.67 ± 0.35 毫米3分别在第 12 周时的 NS 组和 VS 组 (P = .54)。组织学半定量检查在任何时间点均未发现明显的组间差异。结论：在大鼠 ACLR 模型中，将移植物预浸泡在万古霉素中，表明对短期和中期生物力学、放射学和组织学研究没有明显的不利影响。临床相关性：研究结果提供了为外科医生考虑这种技术时提供指导。