Barley leaves (BLs) naturally contained abundant phenolics, most of which are hardly completely released from food matrix during gastrointestinal digestion. Superfine grinding (SFG) and high hydrostatic pressure (HHP) are generally used to treat the functional plants due to their effectiveness to cell wall-breaking and improvement of nutraceutical bioavailability. Thus, this study investigated the synergistic effects of SFG and HHP (100, 300, 500 MPa/20 min) on the bioaccessbility of typical phenolics in BLs during the simulated digestion. The results demonstrated that the highest bioaccessbility (40.98%) was found in the ultrafine sample with HHP at 500 MPa. CLSM and SEM confirmed SFG led to microstructurally rapture of BLs. Moreover, the recovery index of ABTS radical scavenging activity and FRAP of HHP-treated ultrafine and fine BLs samples maximumly increased by 53.62% and 9.61%, respectively. This study is expecting to provide the theoretical basis to improve the consumer acceptance of BLs.

中文翻译：

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超微粉碎和高静水压对大麦叶多酚含量、分布、消化行为和抗氧化活性的协同影响


大麦叶（BL）天然含有丰富的酚类物质，其中大部分在胃肠道消化过程中很难完全从食物基质中释放出来。超细研磨（SFG）和高静水压（HHP）通常用于处理功能性植物，因为它们可以有效地破坏细胞壁并提高营养保健品的生物利用度。因此，本研究研究了模拟消化过程中 SFG 和 HHP（100、300、500 MPa/20 分钟）对 BL 中典型酚类生物可及性的协同效应。结果表明，在 500 MPa 的 HHP 下的超细样品中发现了最高的生物可及性 (40.98%)。 CLSM 和 SEM 证实 SFG 导致 BL 的微观结构破裂。此外，经HHP处理的超细和细BLs样品的ABTS自由基清除活性和FRAP恢复指数最大分别增加了53.62%和9.61%。本研究期望为提高消费者对BL的接受度提供理论依据。