In the present study, the highly efficient electrochemiluminescence (ECL) luminophores of Zr-based metal–organic frameworks (MOFs) coordinating with the amino group of luminol (Zr–Lu–MOFs) were developed for fabricating a sensing platform. The ECL emission of a Zr–Lu–MOF/HO system with strong and stable ECL signals was triggered through cyclic voltammetry. Adenosine triphosphate (ATP) was firmly assembled on Zr–Lu–MOF because of the high affinity of Zr toward phosphate groups. ATP was detected using gallic acid (GA)-capped Au (GA@Au) nanoparticles combined with an ATP aptamer. The GA@Au nanoparticles quenched the ECL behavior of the luminol/HO system, and the quenching mechanism was ascribed to the GA scavenging superoxide radical (O) that was generated via the electron redox reaction in HO. Under optimal experiments, an ECL biosensor with a linear range of 0.100–1000 nM and a detection limit of 0.0353 nM (S/N = 3) was designed for the selective and sensitive detection of ATP. Thus, the proposed ECL biosensor exhibited good performance with high stability and acceptable fabrication reproducibility, providing a valuable strategy for clinical diagnostics and therapeutics.

中文翻译：

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使用没食子酸封端的金纳米粒子清除 ROS，对鲁米诺掺杂的 Zr-MOF 进行电化学发光猝灭，用于 ATP 检测

在本研究中，开发了与鲁米诺氨基（Zr-Lu-MOF）配位的Zr基金属有机框架（MOF）的高效电化学发光（ECL）发光体，用于制造传感平台。通过循环伏安法触发 Zr-Lu-MOF/H2O 系统的 ECL 发射，具有强而稳定的 ECL 信号。由于 Zr 对磷酸基团的高亲和力，三磷酸腺苷 (ATP) 牢固地组装在 Zr-Lu-MOF 上。使用没食子酸 (GA) 封端的金 (GA@Au) 纳米颗粒与 ATP 适配体结合检测 ATP。 GA@Au纳米粒子猝灭了鲁米诺/H2O体系的ECL行为，猝灭机制归因于GA清除H2O中电子氧化还原反应产生的超氧自由基(O)。在最佳实验下，设计了线性范围为0.100-1000 nM、检测限为0.0353 nM（S/N = 3）的ECL生物传感器，用于选择性和灵敏地检测ATP。因此，所提出的ECL生物传感器表现出良好的性能、高稳定性和可接受的制造再现性，为临床诊断和治疗提供了有价值的策略。