It is of utmost importance to develop a simple, sensitive, and dependable technique for detecting kanamycin (Kana). Utilizing MIL-101@AuNP nanohybrids and DNA hydrogel, we devised a dual-mode aptasensor for Kana detection employing surface-enhanced Raman spectroscopy (SERS) and a colorimetric method. The MIL-101@AuNP nanohybrids displayed excellent peroxidase-like and SERS activities. In the existence of Kana, the aptamer specifically bound to Kana, releasing complementary DNA strands (cDNA). Employing free cDNA as a primer, circular DNA templates were generated for the rolling circle amplification (RCA) reaction. DNA hydrogel network formed via the physical entanglement and hybridization of two ultralong single-strand DNA chains from the double RCA-generated, capturing MIL-101@AuNP nanohybrids, thereby enhancing the SERS signal of the DNA hydrogel. Concurrently, the absorbance signal of 3,3′,5,5′-tetramethylbenzidine (TMB)-HO system catalyzed by unfished MIL-101@AuNP was weakened. The dual-mode aptasensor, employing SERS and colorimetric methods, quantitatively detected Kana within concentration ranges of 0.003–300 ng/mL and 0.03–100 ng/mL, respectively, with detection limits (LOD) of 0.001 ng/mL and 0.018 ng/mL, respectively. Importantly, this method was applied to detect Kana-spiked milk samples, yielding satisfactory spiked recoveries. The proposed approach combines the advantages of SERS and colorimetric methods, offering high sensitivity, reliability, and broad applicability, thereby presenting a novel thinking for constructing multimode sensing platforms.

中文翻译：

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表面增强拉曼散射和比色双模式适体传感器，用于基于 DNA 水凝胶网络捕获 MIL-101@AuNP 纳米杂化物的卡那霉素超灵敏检测


开发一种简单、灵敏且可靠的卡那霉素 (Kana) 检测技术至关重要。利用 MIL-101@AuNP 纳米杂化物和 DNA 水凝胶，我们设计了一种采用表面增强拉曼光谱 (SERS) 和比色法进行假名检测的双模式适体传感器。 MIL-101@AuNP 纳米杂化物表现出优异的类过氧化物酶和 SERS 活性。在 Kana 存在的情况下，适配体特异性地与 Kana 结合，释放互补 DNA 链 (cDNA)。使用游离 cDNA 作为引物，生成用于滚环扩增 (RCA) 反应的环状 DNA 模板。 DNA水凝胶网络通过双RCA生成的两条超长单链DNA链的物理缠结和杂交形成，捕获MIL-101@AuNP纳米杂化物，从而增强DNA水凝胶的SERS信号。同时，未捕捞的MIL-101@AuNP催化的3,3',5,5'-四甲基联苯胺(TMB)-HO系统的吸光度信号减弱。双模式适配体传感器采用SERS和比色法，分别在0.003-300ng/mL和0.03-100ng/mL浓度范围内定量检测假名，检测限（LOD）为0.001ng/mL和0.018ng/mL。分别为毫升。重要的是，该方法用于检测加标假名的牛奶样品，获得了令人满意的加标回收率。该方法结合了SERS和比色法的优点，具有高灵敏度、可靠性和广泛的适用性，从而为构建多模式传感平台提供了一种新颖的思路。