Norfloxacin (NOR) is widely used in medicine and animal husbandry, but its accumulation in the environment poses a substantial threat to ecological and human health. Traditional physical, chemical, and rudimentary biological methods often fall short in mitigating NOR contamination, necessitating innovative biological approaches. This study proposes an engineered bacterial consortium found in marine sediment as a strategy to enhance NOR degradation through inter-strain co-metabolism of diverse substrates. Strategically supplementing the engineered bacterial consortium with exogenous carbon sources and metal ions boosted the activity of key degradation enzymes like laccase, manganese peroxidase, and dehydrogenase. Iron and amino acids demonstrated synergistic effects, resulting in a remarkable 70.8% reduction in NOR levels. The innovative application of molecular docking elucidated enzyme interactions with NOR, uncovering potential biodegradation mechanisms. Quantitative assessment reinforced the efficiency of NOR degradation within the engineered bacterial consortium. Four metabolic routes are herein proposed: acetylation, defluorination, ring scission, and hydroxylation. Notably, this study discloses distinctive, co-operative metabolic pathways for NOR degradation within the specific microbial community. These findings provide new ways of understanding and investigating the bioremediation potential of NOR contaminants, which may lead to the development of more sustainable and effective environmental management strategies.

中文翻译：

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定量评估、分子对接和新的代谢途径揭示了诺氟沙星生物降解与环境影响之间的相互作用机制


诺氟沙星（NOR）广泛应用于医药和畜牧业，但其在环境中的积累对生态和人类健康构成重大威胁。传统的物理、化学和基本的生物方法往往不足以减轻 NOR 污染，因此需要创新的生物方法。这项研究提出了在海洋沉积物中发现的工程细菌群落，作为通过不同底物的菌株间共代谢来增强 NOR 降解的策略。用外源碳源和金属离子战略性地补充工程细菌聚体，可以提高漆酶、锰过氧化物酶和脱氢酶等关键降解酶的活性。铁和氨基酸表现出协同效应，使 NOR 水平显着降低 70.8%。分子对接的创新应用阐明了酶与 NOR 的相互作用，揭示了潜在的生物降解机制。定量评估增强了工程细菌群内 NOR 降解的效率。本文提出了四种代谢途径：乙酰化、脱氟、断环和羟基化。值得注意的是，这项研究揭示了特定微生物群落内 NOR 降解的独特、协同代谢途径。这些发现为理解和研究 NOR 污染物的生物修复潜力提供了新的方法，这可能会导致制定更可持续和更有效的环境管理策略。