SummaryProtein complexes from edible oyster mushrooms (Pleurotus sp.) composed of pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) exert toxicity in feeding tests against Colorado potato beetle (CPB) larvae, acting through the interaction with insect‐specific membrane sphingolipid. Here we present a new strategy for crop protection, based on in planta production of PlyA2/PlyB protein complexes, and we exemplify this strategy in construction of transgenic potato plants of cv Désirée. The transgenics in which PlyA2 was directed to the vacuole and PlyB to the endoplasmic reticulum are effectively protected from infestation by CPB larvae without impacting plant performance. These transgenic plants showed a pronounced effect on larval feeding rate, the larvae feeding on transgenic plants being on average five to six folds lighter than larvae feeding on controls. Further, only a fraction (11%–37%) of the larvae that fed on transgenic potato plants completed their life cycle and developed into adult beetles. Moreover, gene expression analysis of CPB larvae exposed to PlyA2/PlyB complexes revealed the response indicative of a general stress status of larvae and no evidence of possibility of developing resistance due to the functional inactivation of PlyA2/PlyB sphingolipid receptors.

中文翻译：

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来自食用蘑菇的蛋白质复合物作为马铃薯可持续的鞘翅目害虫保护剂

摘要来自食用平菇的蛋白质复合物（侧耳属sp.）由侧耳溶菌素 A2 (PlyA2) 和侧耳溶菌素 B (PlyB) 组成，在针对科罗拉多马铃薯甲虫 (CPB) 幼虫的饲养试验中发挥毒性，通过与昆虫特异性膜鞘脂相互作用发挥作用。在这里，我们提出了一种新的作物保护策略在普兰塔PlyA2/PlyB 蛋白复合物的生产，我们在 cv Désirée 转基因马铃薯植物的构建中举例说明了这一策略。其中 PlyA2 定向到液泡而 PlyB 定向到内质网的转基因可有效保护其免受 CPB 幼虫的侵染，而不影响植物性能。这些转基因植物对幼虫摄食率显示出显着影响，以转基因植物为食的幼虫平均比以对照植物为食的幼虫轻五到六倍。此外，只有一小部分（11%–37%）以转基因马铃薯植物为食的幼虫完成了它们的生命周期并发育成成年甲虫。此外，对暴露于 PlyA2/PlyB 复合物的 CPB 幼虫的基因表达分析揭示了表明幼虫一般应激状态的反应，并且没有证据表明由于 PlyA2/PlyB 鞘脂受体功能失活而可能产生抗性。